蝙蝠葛活性成分对人白血病K562及肺癌A549细胞株的抑制增殖作用

Effects of growth inhibition of active components of menispermum dauricum on the human leukemia K562 and lung cancer A549 cell lines

[目的]探讨蝙蝠葛活性成分对体外人白血病K562及肺癌A549细胞株的抑制增殖作用.[方法]利用倒置显微镜及HE染色方法观察给予20mg/L蝙蝠葛活性成分处理后的体外人白血病K562细胞株的形态学变化;采用血清药理学实验方法观察含药(20mg/L蝙蝠葛活性成分)血清对体外人肺癌A549细胞株的抗增殖作用.[结果]倒置显微镜观察结果显示,给予20mg/L蝙蝠葛活性成分后早期K562细胞出现凋亡形态学变化,细胞膜鼓泡,凋亡小体形成,晚期K562细胞出现膜破裂坏死.HE染色结果显示,给予20mg/L蝙蝠葛活性成分后,K562细胞出现典型凋亡形态学变化,凋亡小体形成,细胞核浓缩呈蓝黑色,胞浆呈淡红色,核染色质浓缩呈碎块状.血清药理学实验结果显示,终质量分数分别为10%,20%,25%的灭活的含药大鼠血清对人肺癌A549细胞株的增殖抑制率分别为29.03%,30.55%,41.67%,与空白对照组比较明显增高(P<0.05).[结论]蝙蝠葛活性成分对人白血病K562细胞株具有抑制增殖和诱导凋亡的作用;含蝙蝠葛活性成分血清对体外人肺癌A549细胞株具有抑制增殖作用.

OBJECTIVE To investigate the growth inhibition effects of active components of menispermum dauricum on the human leukemia K562 and lung cancer A549 cell lines.METHODSInverted microscope and HE staining were used to observe the morphological changes of leukemia K562 cells which were given 20 mg/L active components of menispermum dauricum.Serum pharmacology assay was used to measure the growth inhibition rate of human lung cancer A549 cells which were treated with the serum containing20 mg/L active components of menispermum dauricum.RESULTS Under inverted microscope the leukemia K562 cells showed the morphological changes of apoptosis such as the cell membrane bubbing and the formation of the apoptotic bodies at the early stage after treated with 20 mg/L of active components of menispermum dauricum;while the K562 cells showed membrane rupture and necrosis at the later stage.The results of HE staining showed that the cells which were treated with 20 mg/L of active components of menispermum dauricum exhibited the typical morphological changes of apoptosis,the formation of the apoptotic bodies,the blue and black concentrated nucleus,the pompadour cytoplasm and concentrated chromatin in pieces.The results of serum pharmacology assay demonstrated that the growth inhibition rates of A549 cells treated with the 10%,20% and 25% final mass fraction inactived serum were 29.03%,30.55% and 41.67% respectively.Compared with the control group,the differences were significant（P0.05）.CONCLUSION The active components of menispermum dauricum had the effect of inhibiting proliferation and inducing apoptosis on human leukemia K562 cells.The serum containing active components of menispermum dauricum could inhibit the growth of human lung cancer A549 cells.