肿瘤患者细胞免疫功能的影响因素分析

Analysis of influencing factors of cellular immune function in cancer patients

目的:旨在探讨各相关因素对肿瘤患者的细胞免疫功能的影响。方法:采用我院检验科的BD FACSCantoⅡ流式细胞仪分析2016年6月至2017年4月期间67例肺癌、胃癌及结直肠癌肿瘤患者体内CD4^+、CD8^+细胞计数及CD4^+/CD8^+,将CD4^+细胞计数或CD4^+/CD8^+低于正常范围最低值的患者定义为其细胞免疫功能低下,使用SPSS19.0统计分析软件全方位分析各相关因素与CD4^+、CD8^+细胞计数、CD4^+/CD8^+及细胞免疫功能低下之间的关系,最后客观地讨论出可能会受到影响的相关因素。结果:男性及年龄≤60岁的患者的CD4^+、CD8^+细胞计数和CD4^+/CD8^+比值均高于女性及>60岁的患者,且总蛋白(Total protein,TP)降低组、白蛋白(Albumin,ALB)降低组、血红蛋白(Hemoglobin,HGB)降低组及癌胚抗原(Carcinoembryonic antigen,CEA)升高组的CD4^+、CD8^+细胞计数和CD4^+/CD8^+比值均低于各分组的正常组,其中性别组、TP组及CEA组的细胞计数具有显著性差异(P<0.05);单因素分析的细胞免疫功能低下的患者中,CEA升高组的比例低于CEA正常组,其差异具有显著性(P<0.05);但在多因素分析中并无具有显著差异性的相关因素(P>0.05)。结论:随时关注患者营养状况并及时进行纠正能有效地保护患者的细胞免疫功能,这也在临床的抗肿瘤免疫过程中发挥着重要作用。

Objective：To explore the impact of various factors on the cellular immune function of cancer patients.Methods：The CD4＋,CD8＋cells absolute value and the ratio of CD4＋and CD8＋cells in 67 blood specimens collected from patients with lung cancer,gastric cancer or colorectal cancer during June 2016 to April 2017 were analyzed by BD FACSCantoII flow cytometry.And then the patients were diagnosed with low cellular immune function when the absolute value and the ratio of CD4＋and CD8＋cells is lower than the normal range.The relationship between CD4＋,CD8＋cell count,CD4＋/CD8＋and low cellular immune function were analyzed by SPSS19.0 statistical analysis software.Finally,the factors that might be affected were discussed objectively.Results：The CD4＋,CD8＋cell count and CD4＋/CD8＋ratio among male younger than 60 years were higher than those in female older than 60 years.And the CD4＋,CD8＋ cells absolute value and the ratio of CD4＋ and CD8 ＋ cells in TP＋group,ALB reduction group,HGB reduction group and CEA group were lower than the normal group.The cells count was significantly different in sex group,TP group and CEA group（P〈0.05）.In patients with low immunologic function of single factor analysis,the proportion of CEA was lower than the normal group of CEA,and the difference was significant（P〈0.05）.However,there was no significant difference by multifactor analysis（P〈0.05）.Conclusion：Concerned about the nutritional status of patients and timely correction can effectively protect the patient′s cellular immune function,which is also in the clinical anti-tumor immune process plays an important role.