雄激素和雌激素以及催乳素性相关激素受体在喉鳞状细胞癌中的表达及预后影响

The expression and clinical progression of androgen,estrogen and prolactin receptor in laryngeal squamous cell carcinoma

目的:探讨在喉鳞状细胞癌(LSCC)中,包括雄激素受体(AR)和雌激素受体(ER)在内的性激素受体,其表达情况和作用的异同以及机制。结合催乳素受体(PRLR)的表达情况,共同分析3个受体对于肿瘤的预后影响。方法:本研究针对112例LSCC患者(男95例、女17例)的肿瘤组织及邻近的正常组织进行免疫组织化学和逆转录聚合酶链反应(RT-PC)分析。结果:在肿瘤组织中的AR,ER-α以及PRLR的表达,其在蛋白水平和mRNA水平均远高于邻近的正常组织(P>0.05)。在以免疫组织化学结果为基础的进一步分析中发现:肿瘤组织中ER-α(P=0.06)以及PRLR(P=0.02)的相对高表达预示着LSCC患者术后生存率不高。此外,ER-α的高表达和PRLR的高表达比较差异有统计学意义(r=0.802,P=0.04),表明LSCC各激素受体表达可能存在相互影响。通过对组织样本的研究,发现激素受体表达与临床肿瘤病变情况和总体生存数据有密切关系。结论:性相关激素受体表达情况对LSCC的发生发展有其特定的影响。

Objective：The aim of this study is to analyze the different expression and function of androgen receptors（AR）and estrogen receptor（ER-α）in laryngeal squamous cell carcinoma（LSCC）.In combination with the expression of prolactin receptor（PRLR）,we analyzed the prognostic impact of three receptors on the laryngeal carcinoma.Method：In this study,immunohistochemistry and reverse transcription polymerase chain reaction（RTPC）analysis were performed on the tumor tissues and adjacent normal tissues in 112 LSCC patients（95 males and 17 females）.We found that hormone receptor expression is closely related to the clinical tumor lesions and overall survival data.Result：The expression of AR,ER-αand PRLR in tumor tissues were much higher than those in adjacent normal tissues（P〈0.05）at both protein and mRNA levels.The higher PRLR level indicate poor survival in LSCC patients（P=0.02）,while higher ER-αexpression could influence the survival with considerable trend toward significance（P=0.06）.Furthermore,the higher expression of ER-αin tumours were corresponding with PRLR cytoplasmic higher level expression（r=0.802,P=0.04）.This mutual promoted effect between ER-αand PRLR possibly suggests potential mechanisms among those sex related hormone receptors in laryngeal cancer.Conclusion：It has become increasingly credible that the sex related hormone receptors play an important role in the development of LSCC.