不同提取方法对米邦塔仙人掌粗多糖体外抗氧化性的影响

Effect of different extraction methods on the antioxidant activity of crude polysaccharide from Opuntia Milpa Alta Haw in vitro

目的:研究不同提取方法对米邦塔仙人掌粗多糖体外抗氧化活性的影响。方法:采用热水提法、酶法和超声波法分别提取米邦塔仙人掌粗多糖,苯酚-硫酸法测定粗多糖纯度,以抗肝组织自发性脂质过氧化能力、清除羟基自由基能力、清除超氧阴离子能力、清除1,1-二苯基-2-苦苯肼自由基能力、总还原能力、总抗氧化能力6种方法作为体外抗氧化能力评价指标。结果:酶法提取的粗多糖得率最高,为10.14%;超声波法提取的粗多糖纯度最高,为47.62%;酶法和超声波法提取的粗多糖各项体外抗氧化指标的活性均高于热水提法。结论:米邦塔仙人掌粗多糖的体外抗氧化活性强弱与粗多糖的提取方法有关,酶法和超声波法提取的粗多糖具有较好的抗氧化活性。

Objective： To investigate the effect of extraction methods on the antioxidant activity of crude polysaccharide from Opuntia Milpa Alta Haw in vitro.Methods： Polysaccharides in fresh Opuntia Milpa Alta Haw were extracted by the methods of hot water extraction,enzymatic extraction and microwave extraction respectively,then use the phenol-sulfuric acid method to determine the purity of polysaccharides.The antioxidant activities of the extracted polysaccharides were analyzed by the antilipid peroxidation on spontaneous hepatic tissue,the clearance of hydroxyl radical,superoxide anion and DPPH·,total reduction,total antioxidant capacity. Results： The polysaccharides prepared by enzymatic extraction had the highest extraction yield 10.14%,while the microwave extraction had the highest purity 47.62%,enzymatic extraction and microwave extraction had a higher clearance rate than hot water extraction in the whole antioxidant indicators.Conclusion： The antioxidant activity of polysaccharide in vitro was associated with extraction methods,and the polysaccharides extracted by enzymatic extraction had a better antioxidant activity.