金枪鱼鱼骨活性钙对鼠胚胎成骨细胞前体细胞(MC3T3-E1)活力和凋亡作用

Effect of tuna fish bone activated calcium on viability and apoptosis of mouse embryonic osteoblast precursor cells( MC3T3-E1)

目的:以金枪鱼骨为原料,制备柠檬酸-苹果酸钙剂(CMC),研究其对小鼠胚胎成骨细胞前体细胞(MC3T3-E1)的细胞活力、凋亡的影响,并探讨其作用机制。方法:在小鼠胚胎成骨细胞前体细胞(MC3T3-E1)培养液中,分别加入高浓度(1μg/m L)CMC和低浓度(0.1μg/m L)CMC,利用MTT法检测对MC3T3-E1细胞活力的影响,流式细胞仪检测血清饥饿诱导的细胞凋亡。结果:高浓度和低浓度的钙对MC3T3-E1细胞活力均有显著的促进作用,24 h的高钙组的细胞增长率(9.67%)优于低钙组(8.95%);36 h和48 h后低钙组的增长率分别为14.96%和20.33%,明显优于36 h和48 h作用后的高钙组(6.23%和1.73%),两组细胞凋亡率与空白组比较差异较小,且均显著低于对照组(p<0.01)。结论:CMC高钙组和CMC低钙组在一定浓度范围内,能促进MC3T3-E1细胞活力显著增强,并且改善血清饥饿诱导的成骨前体细胞凋亡。

Objective： The aim of this study was to inspect the effect of citric-malic acid calcium（ CMC） on the cell viability and apoptosis of mouse embryonic osteoblast precursor cells（ MC3 T3-E1） was investigated by using tuna bone as raw material,and to explore the mechanism of action between CMC and cells. Methods： A high concentration（ 1 μg/m L） of CMC and a low concentration（ 0.1 μg/m L） of CMC were added to the culture medium of mouse embryonic osteoblast precursor cells（ MC3 T3-E1）,MTT assay was used to detect MC3 T3-E1 cell viability,flow cytometry was used to detect serum starvationinduced apoptosis. Results： It showed that high concentration and low concentration of calcium had a significant effect on the viability of MC3 T3-E1 cells.After incubation for 24 hours,the cell rate（ 9.67%） in the high calcium group was better than that in the low calcium group（ 8.95%）. The proliferation rates of low calcium group were 14.96% and 20.33% at 36 h and 48 h,respectively,which were significantly higher than those in high calcium group（ 6.23% and 1.73%） after 36 h and 48 h. The apoptotic rate of the two groups was significantly lower than that of the blank group（ p 〈 0.01）.Conclusion： CMC high calcium group and CMC low calcium group in a certain concentration range,can promote MC3 T3-E1 cell viability significantly enhanced,and inhibit the serum starvation-induced osteogenic precursor cell apoptosis.