白细胞介素-18在脓毒症血小板减少症中的表达及临床意义

Expression of IL-18 in sepsis-associated thrombocytopenia and its clinical significance

目的检测白细胞介素-18(IL-18)在脓毒症患者及脓毒症相关血小板减少症(TCP)患者外周血中的表达水平并初步探讨其临床意义。方法采用实时定量PCR的方法检测IL-18在脓毒症患者和脓毒症TCP患者外周血单个核细胞中的相对表达情况;采用酶联免疫吸附法检测IL-18在脓毒症患者和脓毒症TCP患者外周血血清中的相对表达情况。结果脓毒症患者外周血单个核细胞以及血清中IL-18的表达量均低于脓毒症TCP患者。结论 IL-18与脓毒症患者血小板减少程度呈负相关,并且极有可能在脓毒症TCP的发病过程中起重要作用。

Objective To investigate the expression of IL-18 in severe sepsis and sepsis-associated throm- boeytopenia and explore its clinical significance. Methods Real-time polymerase chain reaction was applied to de- tect the expression of IL-18 miRNA in 28 samples of sepsis-associated thrombocytopenia patients and 32 samples of severe sepsis. The enzyme-linked immunosorbent assay was applied to detect the concentration of IL-18 in their plasma. Results The miRNA expression of IL-18 in the sepsis-associated thrombocytopenia group was higher than in the severe sepsis group （P=0.015）. The concentration of IL-18 in the severe sepsis-associated thromboeyto- penia group were also higher than those in the severe sepsis group （P=0.034）. Conclusion The expression level of IL-18 is related with the severity of thromboeytopenia in patients with sepsis and is likely to play an important role in the pathogenesis of sepsis-associated thrombocytopenia.