Pseudomonas sp.HS-2降解双酚F及脱氮的特性研究

Research on the characteristics of bisphenol F degradation and denitrification by Pseudomonas sp.HS-2

Pseudomonas sp.HS-2能以双酚F为唯一碳源、NH_4^+-N为唯一氮源进行生长,并通过异养硝化好氧反硝化过程脱氮。在35℃,150 r/min和pH=7.0条件下,考察了菌株HS-2降解双酚F的能力及其脱氮性能。结果表明,菌株HS-2能够降解500 mg/L双酚F。并且在最适碳氮比为20的条件下,双酚F和NH_4^+-N的去除率可分别达到99%和96%。并且发现氨单加氧酶参与了双酚F的降解。氮平衡分析表明,47.7%的初始NH_4^+-N转化为N_2。外加柠檬酸钠或硝态氮对双酚F和NH_4^+-N的去除有明显的促进作用。

Pseudomonas sp. HS-2 can grow by using bisphenol F as sole carbon source and NH4＋-N as sole nitrogen source, and denitrify via the process of heterotrophic nitrification-aerobic denitrification （HN-AD）. Bisphenol F de- gradation and denitrification capabilities of strain HS-2 have been investigated under the following conditions：35℃, 150 r/min and pH 7.0. The results show that strain HS-2 could efficiently degrade 500 mg/L of bisphenol F. Under the optimal C/N ratio of 20, the removing rates of bisphenol F and NH4＋-N can reach 99% and 96% in 48 h, respec- tively. In addition,it is found that during this process, ammonia monooxygenase has participated in BPF degradation. Nitrogen balance analysis demonstrates that 47.7% of initial NH4＋-N is converted into N2. The additional sodium cit- rate and NO3_ -N have remarkable facilitating effects on the removal of bisDhenol F and NH4＋-N.