桂枝茯苓对子宫内膜异位症大鼠异位内膜组织生存素和半胱氨酸蛋白-3表达的影响

Effect of Guizhi Fuling on the expression of Survivin and Caspase-3 in ectopic endometrium of endometriosis rats

目的研究桂枝茯苓对子宫内膜异位症(EMs)大鼠异位内膜组织生存素(Survivin)和半胱氨酸蛋白-3(Caspase-3)表达的影响。方法自体移植法建立EMs模型,随机分为模型组、对照组和低、中、高剂量实验组,每组15只。模型组均予以0.9%NaCl 2 mL;对照组予以0.52 mg·mL^(-1)米非司酮混悬液2 mL;低、中、高剂量实验组分别予以29,58和116 mg·mL^(-1)桂枝茯苓混悬液2 mL。5组大鼠均予以灌胃给药,每天1次,连续用药28 d。取子宫内膜组织,用免疫组织化学法检测Survivin和Caspase-3的表达量。结果模型组、对照组和低、中、高剂量实验组的Survivin阳性表达率分别为92.86%,26.67%,60.00%,33.33%和28.57%,Caspase-3阳性表达分别为14.29%,86.67%,46.67%,73.33%和85.71%,对照组和低、中、高剂量组的上述指标与模型组比较,差异均有统计学意义(均P<0.05)。结论桂枝茯苓治疗EMs的作用机制可能是通过下调Survivin在EMs中的高表达,激活细胞中的Caspase-3活化途径,从而抑制异位内膜的增长。

Objective To study the effect of Guizhi Fuling on the expression of Survivin and Caspase-3 in endometriosis（ EMs） rats.Methods The EMs model was built by autologous transplantation. The rats were randomly divided into model group,control group,low-dose,middle-dose and high-dose test groups,with 15 rats per group. Model group was treated with 0. 9% NaCl 2 mL. Control group was given 0. 52 mg·mL^（-1） mifepristone suspension 2 mL. Low-dose,middle-dose and high-dose test groups were treated with 29,58 and 116 mg·mL^（-1） Guizhi Fuling suspension 2 mL,respectively. Five groups rats were given drugs by intragastric administration,qd for 28 days. The expressions of Survivin and Caspase-3 were detected by immunohistochemistry.Results In the model group,control group,low-dose,middle-dose and high-dose test groups,the positive expression rates of Survivin were92. 86%,26. 67%, 60. 00%, 33. 33% and 28. 57%, the positive expression rates of Caspase-3 were 14. 29%,86. 67%,46. 67%,73. 33% and 85. 71%. Compared with model group,the above indicators in the control group and low,medium and high dose groups had statistically significant differences（ all P 0. 05）. Conclusion Themechanism of Guizhi Fuling in the treatment of EMs may be the high expression of Survivin and the activation of cells in Caspase-3 activation pathway,which induces ectopic endometrial cells atrophy,necrosis and apoptosis,thus inhibiting the growth of ectopic endometrium.