胍丁胺通过细胞因子介导多器官功能衰竭小鼠保护作用的研究

Protective effects of AGM through inflammatory cytokines mediated on MODS mice

目的:通过探讨胍丁胺(AGM)对炎症因子表达水平的影响,研究胍丁胺对酵母多糖(ZYM)诱导小鼠多器官功能衰竭(MODS)的保护作用。方法:小鼠腹腔注射ZYM+AGM,建立ZYM诱导的炎症模型,同时设立空白组、AGM组、ZYM组,观察各组建模前后小鼠进食、白细胞计数、心率等以确定造模是否成功。造模成功后对各组小鼠的肝功能、肾功能、心肌酶等生化指标进行检测;并通过q PCR和ELISA方法检测血液中肿瘤坏死因子(TNF-α)、白细胞介素1β(IL-1β)、白细胞介素6(IL-6)、IL-10因子的基因和蛋白分泌水平。结果:注射ZYM后的小鼠出现精神不振、活动和进食减少;检测小鼠各脏器功能血清学指标,显示脏器功能出现严重异常。与空白对照组相比,ZYM组、ZYM+AGM组小鼠血清学指标均明显增高;并伴随炎症因子TNF-α、IL1β、IL-6、IL-10明显升高(P<0.05)。与ZYM组相比,ZYM+AGM组各脏器功能血清学指标降低,炎症因子TNF-α、IL-1β、IL-6明显下降(P<0.05),IL-10无明显差异(P>0.05),而小鼠精神、活动和进食等无明显的改变。结论:腹腔注射500 mg/kg的ZYM能够成功构建小鼠MODS模型,AGM通过降低炎症因子的释放,对MODS小鼠各器官功能起到一定的保护作用。

Objective： To explore the protective effect of agmatine on mice with multiple organ failure（ MODS） induced by yeast polysaccharide（ ZYM） on the expression of inflammatory factors. Methods： ZYM induced inflammation model was established by intraperitoneal injection of ZYM in mice. All mice were divided into blank group,ZYM group and ZYM＋AGM group. The mice feeding,white cell count,heart rate and so on were observed before and after the modeling to determine whether the model was successful. The liver function of mice,renal function,myocardial enzymes and other biochemical indicators were detected after the success of the model;and through the q PCR and ELISA method for detection of blood tumor necrosis factor alpha（ TNF-alpha）,interleukin 1 beta（ IL-1 beta）,interleukin 6（ IL-6）,IL-10 gene and protein secretion level. Results： After the injection of ZYM,mice looked disorganized,activity and reduce consumption; the functional serological indexes of various organs of the mice were detected,which showed that the function of the viscera was serious. Compared with the blank control group,the serum parameters of ZYM group and ZYM＋AGM group were significantly higher,and the inflammatory factors TNF-α,IL-1,IL-6 and IL-10 were significantly increased（ P〈0. 05）. Compared with ZYM group,ZYM＋AGM serum markers of organ function decreased,inflammatory factor TNF-α,IL-1β,IL-6 decreased significantly（ P〈0. 05）,while there was no significant difference in IL-10（ P〉0. 05）; and the mouse spirit,eating and activity had no significant change. Conclusion： Intraperitoneal injection of 500 mg/kg ZYM can successfully construct a model of MODS,AGM by reducing the release of inflammatory factors,play a protective role in the function of various organs of MODS mice.