定坤丹对PCOS模型大鼠TGF-β1、CTGF和AR表达的影响

Effects of Ding Kundan on expression of TGF-β1,CTGF and AR in PCOS model rats

目的:研究定坤丹对PCOS模型大鼠TGF-β1、CTGF和AR表达的影响,探讨定坤丹治疗PCOS的作用机制。方法:应用高、中、低剂量定坤丹(4.5、2.25、1.125 g/kg)干预DHEA诱导的PCOS大鼠模型,采用ELISA、免疫组化、RT-PCR及Western blot检测各组大鼠血清激素水平、TGF-β1、CTGF和AR mRNA及蛋白的表达。结果:与模型组比较,定坤丹高、中、低剂量组E2、LH及T均下降,FSH则升高,且差异均具有统计学意义;TGF-β1、CTGF和AR mRNA及蛋白表达均下降,且差异均具有统计学意义。结论:定坤丹对PCOS大鼠有一定治疗作用,其机制可能是通过调节卵巢组织中TGF-β1、CTGF和AR的表达水平。

Objective： To investigate the effect of Ding Kundan on the expression of TGF-β1,CTGF and AR in PCOS model rats,and to explore its treat mechanism on PCOS. Methods： The PCOS model rats induced by DHEA were intervened with high,medium and low dose of Ding Kundan（ 4. 5,2. 25,1. 125 g/kg）,ELISA,immunohistochemistry,RT-PCR and Western blot were used to detect serum hormone levels,mRNA and protein expression of TGF-β1,CTGF and AR in each group of rats. Results： Compared with model group Ding Kundan high,medium and low dose group of E2,LH and T were decreased,while FSH was increased. And the differences were statistically significant. Protein expression and mRNA of TGF-β1,CTGF and AR were decreased,and the differences were statistically significant. Conclusion： Ding Kundan shows significantly therapeutic efficacy on PCOS rats,and its possible mechanism is related to regulating expression levels of TGF-β1,CTGF and AR in ovarian tissue.