山东栽培豆与野生豆抗裂荚基因SHAT1-5的克隆及序列分析

Cloning and Sequence Analysis of Pod Shattering Resistant Gene SHAT1-5 from Cultivated and Wild Soybeans in Shandong Province

大豆的裂荚性是影响其产量的重要因素之一,因此栽培豆中豆荚开裂特性的遗传学机制成为国内外研究的热点。本研究以山东栽培豆(山宁11、山宁17、齐黄34、齐黄35和鲁0305-1)及山东野生豆(东营野生豆1、2号和山东野生豆1、2号)作为试验材料,根据已有报道,通过PCR扩增克隆了一个大豆抗裂荚基因SHAT1-5,并对该基因的序列进行多态性分析。结果显示,山东栽培豆和野生豆在SHAT1-5基因区约1 599 bp位置处,各自出现不同倍数的TAA重复。山东栽培豆与野生豆在SHAT1-5基因启动子上游4 kb关键调控区极度保守,栽培豆中不存在20 bp的缺失,这些结果与前人报道的东北栽培豆和野生豆中的情况有所不同。这表明,山东栽培豆与野生豆的裂荚性可能受其他基因的调控。

Pod shattering is one of the important factors affecting soybean yield. Therefore,the genetic mechanism of pod shattering trait in cultivated soybean has become a hot spot at domestic and overseas. In this research,the cultivars including Shanning 11,Shanning 17,Qihuang 34 and Lu 0305-1,and the wild germplasms including Dongying wild soybean 1 and 2 and Shandong wild soybean 1 and 2 were used as experimental materials. According to the previous reports,we cloned a pod shattering resistant gene SHAT1-5 through PCR amplification,and analyzed its DNA polymorphism. The results showed that SHAT1-5 appeared different multiples of TAA repeats at 1599 bp location in Shandong cultivated and wild soybeans,respectively. The key regulation region of about 4 kb in upstream of SHAT1-5 promoter was extremely conservative,and no deletion of 20 bp was found in Shandong cultivated soybean. These results are different from the previous reports,indicating that the pod shattering trait of Shandong cultivated soybean and wild soybean may be regulated by other genes.