ICP-MS测定全血中5种微量元素及其参考区间的建立

Determinations of 5 kinds of trace elements by ICP-MS and establishment of reference intervals

目的建立同时测定全血中铁(Fe)、镁(Mg)、钙(Ca)、锌(Zn)、铜(Cu)的电感耦合等离子体质谱(ICP-MS)方法。方法将全血样本混匀后用含Triton 100的稀硝酸溶液进行50倍稀释,以锗(Ge)元素为内标,建立ICP-MS检测全血Fe、Mg、Ca、Zn、Cu的方法。收集495名体检健康者全血样本,按性别和年龄(≤16岁和>16岁)分别分组比较并建立5种元素的参考区间。结果 5种元素的标准曲线线性均良好(r值为0.999 6~1.000 0),批内精密度为0.31%~3.62%,批间精密度为1.37%~7.15%,添加回收率为98.2%~112.7%。正常人群、男女性之间全血Fe、Mg、Ca、Zn、Cu水平差异均无统计学意义(P>0.05),而≤16岁组与>16岁组之间5种元素差异均有统计学意义(P<0.05)。按年龄以x±2s方式建立参考区间:≤16岁者Mg为30.2~43.4 mg/L、Ca为47.2~69.6 mg/L、Fe为320.6~466.0 mg/L、Cu为0.64~1.36 mg/L、Zn为3.1~6.8 mg/L;>16岁者Mg为27.0~43.6 mg/L、Ca为42.6~64.6 mg/L、Fe为275.2~530.3 mg/L、Cu为0.40~1.58 mg/L、Zn为4.3~8.3 mg/L。结论建立的ICP-MS方法可同时测定Fe、Mg、Ca、Zn、Cu,敏感性高,操作简便、稳定,准确度高,可用于临床大样本分析。

ObjectiveTo establish an inductively coupled plasma mass spectrometry（ICP-MS） for the determinations of 5 kinds of trace elements [iron（Fe）,magnesium（Mg）,calcium（Ca）,zinc（Zn） and copper（Cu）] in whole blood. MethodsAfter whole blood samples were fully mixed and diluted to 50 times with the solution containing Triton 100 and dilute nitric acid,adding germanium（Ge） as internal standard,an ICP-MS was established for determining 5 kinds of trace elements. A total of 495 whole blood samples of healthy subjects were collected. According to sex and age（≤16 years old and 〉16 years old groups）,the reference intervals were established. ResultsThe standard curve linearities were good for 5 kinds of trace elements（r=0.999 6-1.000 0）. The within-run precision was 0.31%-3.62%,and the between-run precision was 1.37%-7.15%. The recovery rate was 98.2% -112.7 %. Each element had no statistical signi？cance between males and females（P〉0.05）,but there was statistical signi？cance between ≤16 years old and 〉16 years old groups（P〈0.05）. The reference intervals（x±2s） in ≤16 years old group were 30.2-43.4 mg/L for Mg,47.2-69.6 mg/L for Ca,320.6-466.0 mg/L for Fe,0.64 -1.36 mg/L for Cu and 3.1-6.8 mg/L for Zn,and those in 〉16 years old group were 27.0-43.6 mg/L,42.6-64.6 mg/L,275. 2-530.3 mg/L,0.40-1.58 mg/L and 4.3- 8.3 mg/L,respectively. Conclusions ICP-MS can determine Fe,Mg,Ca,Zn and Cu in whole blood simultaneously,with sensitivity,simple operation,stability and accuracy.