转录调控因子AtWRKY71的克隆及其序列分析

Cloning and Sequential Analysis of Transcription Regulator Factor WRKY71 from Arabidopsis

采用RT-PCR方法,从模式植物拟南芥叶片克隆出转录因子WRKY 71,经过测序及blast同源性分析,结果表明,该序列与GenBank登陆的AtWRKY71序列基本一致,只在起始密码子下游57bp位置由G突变为C,但并不改变氨基酸的编码,说明已经成功克隆转录调控因子AtWRKY71,为后续功能鉴定奠定了基础。

The RT-PCR method was used in this study, Cloning of the transcription factor WRKY 71 from the leaf of Arabidopsis thaliana from the model plant,Sequencing and blast homology analysis, The results show that the sequence is basically consistent with the AtWRKY71 sequence of GenBank landing,The 57 bp position is changed from G to C only in the downstream of the starting codons,But it does not change the encoding of amino acids,It shows that the transcriptional regulator AtWRKY71 has been cloned successfully,and lay the foundation for the follow-un function identification.