基于SCoT标记的福建茶树品种(系)遗传多样性分析

Analysis of Genetic Diversity of Fujian Tea Varieties by SCoT Markers

利用SCoT标记对福建茶树资源进行分析,构建适用于福建茶树资源SCoT-PCR扩增体系。从38条SCoT引物中筛选出的16条多态性引物,构建了55份茶树品种(系)的SCoT标记指纹图谱。对55份材料共扩增出219条条带,多态性条带为216条,平均每条引物扩增出13.8条,多态性比率PPB为93.15%,55份供试材料的遗传相似系数(Genetic similarity,GS)介于0.49~0.85,平均为0.67。SCoT标记分析55份供试材料共两个群体的观测等位基因数Na为1.93,有效等位基因数Ne为1.54,Nei基因多样性为0.32,香农指数Shannon为0.48,遗传分化Gst为0.067,基因流Nm为7.01。在遗传相似系数为0.64处,将55份茶树资源分成2大类。

SCoT-PCR amplification system is constructed to analyze the tea resources in Fujian. Totally 16 polymorphic primers were screened from 38 SCo T primers to construct fingerprints of 55 tea cultivars（lines）. A total of 219 bands were amplified from 55 materials, with 216 polymorphic bands. The average bands amplified by each primer and polymorphic ratio were 13.8 and 93.15%. The Genetic similarity（GS） of 55 tea resources ranged from 0.49 to 0.85, with an average of 0.67. The SCo T marker analysis showed that the observed number of alleles was 1.93 in these two tea groups. The effective number of alleles, Nei gene diversity, Shannon＇s information index, genetic differentiation and the gene flow were 1.54, 0.32, 0.48, 0.067 and 7.01, respectively. When the genetic similarity coefficient was set to 0.64, the tea resources could be divided into two major categories.