超声降解落叶松中阿拉伯半乳聚糖及其活性的研究

Ultrasonic-assisted Degradation of Larch Arabinogalactan and Its Antioxidant Activity

以落叶松[Larix gmelinii(Rupr.)Kuzen]的木屑为原料提取阿拉伯半乳聚糖(AG、松针多糖),然后用超声法对所提取的阿拉伯半乳聚糖进行降解。以降解松针多糖对自由基清除率为检测指标,通过单因素试验考察超声功率、降解温度、降解时间、料液比对松针多糖体外抗氧化活性的影响。再利用正交试验确定的最佳降解条件为:降解温度70℃、降解时间2.0h、料液比1∶250、功率150W。在抗氧化活性方面,松针多糖质量浓度越高,对·OH清除率越高,且降解后比降解前在清除·OH和DPPH上有一定提高。

Arabinogalactan （AG,or larch polysaccharide） was extracted from the sawdust of larch CLarix gmelinii （Rupr. ） Kuzen〕 . Then, the obtained larch polysaccharide was physically degraded by ultrasound-assistance. Thefree radical scavenging ability of decomposed larch polysaccharide was selected as an indicator on the evaluation of the antioxidant activity of larch polysaccharide. Single-factor tests were carried out in the study including ultrasonic power,degradation temperature, degradation time, and liquid-to-solid ratio. The optimum degradation condition was acquired with the analysis of the orthogonal tests as following： the degradation temperature was 70℃ ; the deg-radation time was 2. 0h; the liquid-to-solid ratio was 1 ： 250; the power was 150W. The results of antioxidant activity showed that the scavenging rate of the sample to ·OH would increase with the increase of thetion of the polysaccharide. Further,the degraded arabinogalactan will enhance the ability of scaDPPH compared with the original sample in the field of antioxidant activity.