外源性趋化因子CXC配体12对Hela宫颈癌细胞增殖作用及机制

Exogenous CXC chemokine ligand 12 promotes the proliferation of Hela cervical cancer cells through MAPK/ERK pathway

目的探讨外源性趋化因子CXC配体(L)12对宫颈癌Hela细胞增殖的作用及机制。方法 CCK8实验考察不同浓度和不同时间的外源性CXCL12对宫颈癌Hela细胞增殖的影响;蛋白免疫印迹法检测不同浓度的外源性CXCL12对细胞外调节蛋白激酶(ERK)蛋白表达量的影响。结果 48 h后,浓度40 ng/ml的外源性CXCL12对Hela细胞增殖作用明显高于0 ng/ml(P<0.05);72 h后,浓度20 ng/ml的外源性CXCL12对Hela细胞增殖作用明显高于0 ng/ml(P<0.05),且浓度40 ng/ml时尤其明显(P<0.01);与浓度0 ng/ml外源性CXCL12比较,浓度20 ng/ml和40 ng/ml外源性CXCL12显著上调宫颈癌Hela细胞ERK表达量(P<0.05)。结论外源性CXCL12可经活化丝裂原活化蛋白激酶(MAPK)/细胞外调节蛋白激酶(ERK)通路而促进Hela宫颈癌细胞增殖活性。

Objective To investigate the effect and mechanism of exogenous chemokines ligand （CXCL） 12 on the proliferation of Hela cervical cancer cells. Methods CCK8 experiment was conducted to investigate the effect of different concentrations of exogenous CX- CL12 on the proliferation of Hela cervical cancer cells. The effects of different concentrations of exogenous CXCL12 on the expression of ERK protein were detected by the protein immunoblotting. Results Exogenous CXCL12 at the concentration of 40 ng/ml obviously promoted the proliferation of Hela cells as compared with 0 ng/ml after 48 h incubation （ P〈0.05 ）. After 72 h incubation, exogenous CXCL12 at the con- centration of 20 ng/ml significantly promoted the proliferation of Hela cells as compared with 0 ng/ml （ P〈0.05 ）, and especially the concen- tration of 40 ng/ml （P〈0.01）. Compared with the concentration of 0 ng/ml exogenous CXCL12, the concentration of 20 ng/ml and 40 ng/ml exogenous CXCL12 obviously upregulated the expression of ERK protein in Hela cells. Conclusions Exogenous CXCL12 can pro- mote the proliferation of Hela cervical cancer cells by activating the MAPK/ERK pathway.