5-HTTLPR基因多态性对抑郁症患者超敏C-反应蛋白的影响

Effects of the polymorphsim 5-HTTLPR on the hypersensitive C-reactive protein in patients with depression

目的探讨五羟色胺转运体基因启动子区（5-HTTLPR）基因多态性对抑郁症患者超敏C反应蛋白（hypersensitive C-reactive protein，hs-CRP）的影响。方法运用聚合酶链反应-限制性片段长度多态（PCR-RFLP）测定103例抑郁症患者（研究组）和103例健康对照者（对照组）的5-HTTLPR基因多态性。应用免疫荧光法检测两组hs-CRP的水平，应用汉密尔顿抑郁量表（HAMD）评估抑郁症患者严重程度。分析不同基因型对hs-CRP浓度的影响，分析基因型与hs-CRP的交互作用对抑郁症发病的影响。结果两组5-HTTLPR基因型频率和等位基因频率差异无统计学意义（P＝0.81，0.121）。研究组三种基因型hs-CRP浓度差异有统计学意义（P＝0.007），其中SS型[（8.1±2.7）mg/L]明显高于LS型[（4.9±1.8）mg/L]及LL型[（5.2±1.3）mg/L]（P＝0.002，0.001）。患者组不同基因型间阻滞因子分存在差异（F＝4.637 ，P＝0.033），SS基因型阻滞因子分[（9.3±3.1）分]高于LL型[（6.1±2.7）分]与LS型[（5.8±2.1）分]（P＝0.008，0.007）。Logistic回归分析显示，SS基因型对hs-CRP具有交互作用，两者的交互作用与抑郁症的发病呈正相关，SS基因型与hs-CRP的交互作用与抑郁症的相关性更大（OR＝1.890，95%CI＝1.011～3.396）。结论5-HTTLPR基因SS基因型对抑郁症患者hs-CRP存在叠加效应；基因型与hs-CRP的交互作用与抑郁症的发病有关，SS基因型与hs-CRP的交互作用更易引起抑郁症的发生。

ObjectiveTo explore the influences of serotonin transporter promoter region（5-HTTLPR） polymorphism on hypersensitive C-reactive protein（hs-CRP） in patients with depression.Method5-HTTLPR polymorphism was detected by polymerase chain reactive-restriction fragment length polymorphism（PCR-RFLP） in 103 patients with depression and 103 healthy controls.The severity of depression was evaluated by Hamilton Depression Scale（HAMD）. The hs-CRP level was tested by immunofluorescence.The influence of different genotypes on hs-CRP and the interaction of genotype and hs-CRP on the pathogenesis of depression were analyzed.ResultsThe frequency of genotype and allele in 5-HTTLPR was no statistical significant（P=0.81, 0.121） among the three groups.There were statistically significant differences in hs-CRP concentration （P=0.007） among the three genotypes of the study group, and the concentration of hs-CRP in SS genotype（（8.1±2.7）mg/L） was significantly higher than that in LS（（4.9±1.8）mg/L） and LL genotype（（5.2±1.3）mg/L） （P=0.002, 0.001）. The retardation factor in patients were significantly differences in different genotypes（F=4.637 , P=0.033）. SS genotype（9.3±3.1） was significantly higher than LL（6.1±2.7） and LS genotypes（5.8±2.1） in retardation factor（P=0.008, 0.007）. Logistic regression analysis showed that SS genotype was associated with hs-CRP.The interactive effect was positive related to the morbidity of depression.The correlation of interaction between SS genotype and hs-CRP was greater than LL/LS（OR=1.890, 95%CI=1.011-3.396）.ConclusionSS genotype of 5-HTTLPR has strengthen effect on hs-CRP.The interaction of genotype and hs-CRP affects the onset of depression.The interaction of SS genotype and hs-CRP is more likely to effect the onset of depression.