木糖醇月桂酸单酯的分离纯化及其性质表征

Purification and characterization of xylitol monolaurate

以木糖醇和月桂酸为原料,在固定化脂肪酶的催化下合成木糖醇月桂酸单酯。分别采用硅胶柱层析法、溶剂萃取法对产物进行分离纯化,并采用高效液相色谱法进行纯度分析,核磁共振法(NMR)鉴定目标产物结构。对纯化后的木糖醇月桂酸单酯进行表面性质和抑菌性质表征。结果表明:采用硅胶柱层析法及溶剂萃取法分离纯化后得到的木糖醇月桂酸单酯纯度均达到95%以上,经NMR检测目标产物为1-O-木糖醇月桂酸单酯;其HLB值为9.87,为O/W型乳化剂,对大肠杆菌和金黄色葡萄球菌的最小抑菌浓度(MIC)分别为1.0 mg/m L和0.25 mg/m L。

Xylitol monolaurate was synthesized from xylitol and lauric acid with immobilized lipase as catalyst. The products were isolated and purified by silica gel column chromatography and solvent extraction respectively. The purity of the target product was determined by HPLC, and the structure was identified by NMR. The surface property and antimicrobial property of the purified xylitol monolaurate were charac- terized. The results showed that the purities of xylitol monolaurate obtained by silica gel column chroma- tography and solvent extraction both were above 95 %, the target product was 1 - O - xylitol monolaurate, and its HLB value was 9.87, belonging to the O/W emulsifier. The minimum inhibitory concentrations of xylitol monolaurate against Escherichia coli and Staphylococcus aureus were 1.0 mg/mL and 0.25 mg/mL respectively.