大孔树脂纯化美藤果壳酚类物质的研究

Purification of phenolic compounds from Sacha Inchi shells by macroporous resin

以美藤果壳为原料,用70%乙醇提取得到美藤果壳酚类粗提物,研究大孔树脂纯化美藤果壳酚类物质的工艺条件,对AB-8、HPD300、NKA-9、D101、S-8、DM130 6种型号大孔树脂进行了筛选。结果表明:NKA-9型大孔树脂为纯化美藤果壳酚类物质最佳树脂;NKA-9型大孔树脂纯化美藤果壳酚类物质的最佳工艺条件为上样液质量浓度10 mg/m L、上样速度2.0 m L/min、以70%的乙醇溶液洗脱、洗脱速度1.0 m L/min、洗脱剂体积2.5 BV。在最佳工艺条件下纯化后的美藤果壳酚类物质的总酚含量为73.8%,对比于粗提物中的总酚含量33.2%,提高了1.2倍。

With Sacha Inchi （SI） shells as raw material, the crude phenolic compounds extract was extracted by 70% ethanol. The purification process conditions of phenolic compounds from SI shells by macroporous resins were studied, and six kinds of macroporous resins of AB - 8, HPD300, NKA - 9, D101 ,S -8 and DM130 were screened. The results showed that NKA -9 type resin was the most suitable resin for the purification of SI shell phenolic compounds. The optimal purification process conditions were obtained as follows： mass concentration of sample 10 mg/mL, flow rate of sample 2.0 mL/min, with 70% ethanol as eluent, elution rate 1.0 mL/min, and eluent volume 2.5 BV. Under the optimal conditions, the total phenol content in phenolic compounds from SI shells was 73.8%, which was 1.2 times higher than that in crude extract （33.2%）.