三磷酸胞苷合成酶1在胰腺癌细胞株的表达及其临床意义

Expression of cytidine triphosphate synthetase 1 in pancreatic cancer cell lines and its clinical significance

目的探讨三磷酸胞苷合成酶1（CTPSl）在胰腺癌细胞株中的表达及其与患者临床预后的关系。方法检索GEOProfiles数据库，收集39例术后病理证实为胰腺导管腺癌的病例临床资料，采用Kaplan—Meier法分析胰腺癌患者预后与肿瘤CTPSl表达的关系；并采用qPCR、Westernblot技术检测胰腺癌细胞株中CTPSl表达情况。结果qPCR检测：Swl990、BxPC-3、Pancl、MIA．capa2胰腺癌细胞中CTPSlmRNA的表达量分别为1．00d-0．20、2．92±0．95、4．29±O．14、2．26±0．33，正常胰腺导管上皮细胞HPDE为7．70±0．72（t=-16．19，-11．45，-8．09，-13．12，P〈0．05）。Swl990、BxPC-3、PancI、MIA—Capa2胰腺癌细胞中CTPSI蛋白相对表达量分别为0．40±0．06、0．20±0．09、0．68±0．11、0．48±0．06，HPDE细胞为1．09d-0．12（t=-8．97，-10．52，～4．39，-7．88，P〈0．05）。CTPSl高表达组患者的中位总体生存期（0s）为28个月（95％CI：9．2～46．8个月），而CTPSl低表达组患者的中位0s为13个月（95％CI：8．2—17．8个月）（r=4．02，P〈0．05）。结论CTPSI在胰腺癌细胞株中低表达，且其表达水平与患者生存预后呈正相关。

Objective To investigate the cytidine triphosphate synthetase 1 （CTPS1） expression in pancreatic cancer cell lines and its impaction on prognosis. Methods The GEO profiles database was searched to collect clinical data of 39 patients of pancreatic ductal adenocarcinoma. Kaplan-Meier curve was used to analyze the relationship between the patients＇ prognosis and the expression of CTPS1 . qPCR and Western blot were applied to detect the expression of CTPS1 in 4 pancreatic cancer cell lines. Results The mRNA expression of CTPS1 in Sw1990, BxPC-3, Panel, and MIA-Capa2 was 1.00 ± 0. 20, 2.92±0.95, 4.29±0. 14, and 2.26 ±0.33 （t= -16.19, -11.45, -8.09, -13.12, P〈0.05） when compared to the normal pancreatic ductal epithelial cell line HPDE of 7. 70 ± 0. 72. The relative protein expression of CTPS1 in pancreatic cancer cell lines Sw1990, BxPC-3, Panel, and MIA-Capa2 was 0. 40 ± 0.06, 0.20±0.09, 0.68±0.11, and 0.48±0.06 （t= -8.97, -10.5, -4.39, -7.88, P〈0.05） when compared to HPDE of 1.09 ±0. 12. The overall survival （OS） in patients with higher CTPS1 expression than adjacent tissue was 28 months （95% CI： 9. 2 ±6. 8 months）, and that with lower CTPS1 expression was 13 months （95% CI： 8.2 -17.8 month）,（A-2 =4.02, P〈0.05）. Conclusions crI＇PS1 in the pancreatic cancer cell lines are of low expression, and the level is positively related to patients＇ survival time.