一个水稻新的NAC转录因子OsNAC3功能的初步研究

Identification a new NAC transcription factor OsNAC3 in rice

OsNAC3是水稻NAC转录因子家族的一个成员,受MeJA、干旱、盐及冷胁迫诱导表达。OsNAC3与绿色荧光蛋白GFP的融合蛋白定位在烟草细胞核中。OsNAC3在酵母中具有转录激活活性,且转录激活域位于其C端;酵母双杂交和pull down证实OsNAC3可以形成同源二聚体。采用凝胶阻滞的分析方法,明确OsNAC3在体外能与NAC识别序列NACRS特异结合。研究结果为进一步揭示OsNAC3的生物学功能打下了基础。

OsNAC3 is a member of NAC transcription factor family in rice and induced by MeJA,drought,salt or cold treatments. OsNAC3 and GFP fusion protein was targeted to the nucleus. The OsNAC3 protein showed transactivation activity at its C-terminus in yeast and formed homocomplexes by yeast two-hybrid assay and pull down assay.Electrophoretic mobility shift assays demonstratedthat the recombinant OsNAC3 protein could bind specifically to NAC recognized sequence（ NACRS） in vitro. The results provide some data for further understanding the biological function of OsNAC3.