生物胺丹磺酰氯衍生条件优化研究

Study on the Optimization of Derivative Conditions of Biomidan Sulfonyl Chloride

研究了丹磺酰氯作为衍生试剂同5种生物胺反应的条件,优化了丹磺酰氯的浓度、反应体系的p H值、反应的温度和反应时间。研究结果表明:在腐胺、尸胺、组胺、酪胺和精胺浓度分别为8μg/m L的混合生物胺溶液时,5mg/m L丹磺酰氯丙酮溶液与生物胺底物溶液用量比为1∶1时衍生效果最佳,最佳反应p H值为10.5,最佳反应温度为60℃,最佳反应时间为20min。反应条件的初步确立对于进一步优化反应条件和准确定量生物胺含量具有重要意义。

Derivatization conditions of dansyl chloride concentration, pH value, derivatization temperature and time of 5 kinds of biogenic amines with dansyl chloride were optimized. 8μg/mL biogenie amines of putrescine, cadav- erine, histamine, tyramine spermine and 5mg/mL dansyl chloride in acetone were used. The quantity ratio of dan- syl chloride to biogenic amines of 1：1, derivatization time of 20min, pH value of 10.5 and derivatization tempera- ture of 60℃ were the optimal conditions. The results will be useful for further optimization of derivatization condi- tions and quantification of hiogenie amines.