镁、氧化应激和炎症反应在子痫前期预测中的相关性研究

Correlation study of magnesium,oxidative stress and inflammatory reaction in prediction of preeclampsia

目的评估镁、氧化应激和炎症反应与子痫前期发生的关系。方法选择2013年1月至2016年1月于延安市人民医院产科住院并行剖宫产分娩的45例子痫前期孕妇为子痫前期组(动脉血压为140/90 mm Hg、蛋白尿>0.3 g/24 h),选择同期正常孕妇45例为对照组。通过问卷调查研究食物摄入量进而评估镁的摄入量,通过火焰原子吸收光谱法测定血浆、血清和尿液中镁的含量,同时采用标准方法检测氧化应激和炎症反应标志物。Logistic回归分析子痫前期的预测因子,通过受试者工作特征曲线评价Logistic回归模型的预测性。结果两组镁的摄入量和尿中排泄量比较,差异无统计学意义(P>0.05);但子痫前期组血浆和红细胞中镁的含量高于对照组(P<0.05);血浆镁含量与过氧化氢酶、谷胱甘肽过氧化物酶活性、白细胞介素6和肿瘤坏死因子α水平呈正相关;Logistic回归分析结果显示血浆镁和尿8-异前列腺素与先兆子痫有相关性,灵敏性93%,特异性75%。结论孕妇体内镁、氧化应激和炎症反应因子与子痫前期的发病机制有关,并且血浆镁和尿8-异前列腺素都是子痫前期发生的预测指标。

Objective To assess the relationship between magnesium, oxidative stress and inflammatory response and preeclampsia. Methods 45 pregnant women with preeclampsia undergoing cesarean delivery from Jan. 2013 to Jan. 2016 in People＇s Hospital of Yan＇an were enrolled as preeclampsia group（arterial blood pressure 140/90 mmHg, proteinuria 〉 0. 3 g/24 h）. During the same period, 45 pregnant women without any complications were select as the control group. Through a questionnaire to study food intake and assess magnesium intake, The contents of magnesium in plasma, serum and urine were determined by /lame atomic absorption spectrometry. At the same time, oxidative stress and inflammatory response markers were detected by standard methods. Logistic regression analysis of predictors of preeclampsia and logistic regression models were used to assess the predictive value of the logistic regression model. Results There were no significant differences in magnesium intake and urinary excretion between the two groups （P 〉 0.05）. However, the levels of magnesium in plasma and erythrocyte in preeclampsia group were higher than those in the control group（P 〈 0. 05）. Plasma magnesium was positively correlated with catalase, glutathione peroxidase, interleukin- 6 and tumor necrosis factor - alpha. Regression analysis showed that plasma magnesium and urinary 8 - isoprostane were associated with preeclampsia following 93 % sensitivity and 75 % specificity. Conclusion Magnesium status, oxidative stress and in ammatiun were associated with pathogenesis of preeclampsia. Plasma magnesium and urinary 8 - isoprostane were predictors of preeclampsia.