自体浓缩生长因子对比格犬脂肪干细胞体外增殖及成骨向分化的影响

Effects of concentrated growth factors on proliferation and osteogenic differentiation in Beagle adipose-derived stem cells

目的:分析自体浓缩生长因子(concentrated growth factor,CGF)对比格犬脂肪干细胞(adipose-derived stem cells,ADSCs)体外增殖及成骨向分化的影响。方法:取健康比格犬脂肪组织进行分离、培养ADSCs并鉴定其多向分化潜能,细胞计数试剂盒8(cell counting kit-8,CCK-8)检测细胞增殖。将ADSCs分为CGF组及对照组,对两组细胞进行成骨诱导后分别检测其碱性磷酸酶(ALP)活性,RT-PCR法检测I型胶原(Col-I),Runx2等成骨相关基因表达。结果:CGF组细胞增殖能力明显高于对照组(P<0.05);经成骨诱导的ADSCs在CGF作用下ALP活性明显增强(P<0.05),Col-I,Runx2等成骨相关基因的表达亦明显高于对照组(P<0.05)。结论:CGF能够促进ADSCs体外增殖和成骨向分化。

Objective： To investigate the effect of concentrated growth factor （CGF） on proliferation and differentiation in Beagle adipose-derived stem cells （ADSCs）. Methods： ADSCs were isolated from adipose tissue of healthy Beagles and cultured. The multi-directional differentiation potential of ADSCs was identified. The ADSCs were assigned to a CGF group and a control group. The rate of proliferation was analyzed by CCK-8 assay. The osteogenic differentiation capability was detected by ALP staining after the osteoinduction. Bone formation- related gene expression was detected by RT-PCR. Results： CGF promoted the proliferation of ADSCs in vitro. ADSCs in the CGF group showed higher level of ALP activity than that in the control group （P〈0.05）. CGF stimulated the expression of the genes associated with osteogenesis, such as Col-I and Runx2. Conclusion： CGF can promote the proliferation and osteogenic differentiation in ADSCs in vitro.