转化生长因子β1和骨形态发生蛋白-9在人骨不连组织中的表达及其临床意义

Expression of transforming growth factor 1 and bone morphogenetic protein 9 in human nonunion tissues and its clinical significance

目的:检测肥大性骨不连组织与萎缩性骨不连组织中转化生长因子β1和骨形态发生蛋白-9表达的差异,评价两种骨不连组织在成骨潜能上的区别。方法:收集2010年至2014年中南大学湘雅二医院和上海交通大学医学院苏州九龙医院骨科住院的肥大性和萎缩性骨不连患者各20例。利用免疫组织化学SP二步法及病理图像分析软件IPP6.0对骨不连断端间组织转化生长因子β1和骨形态发生蛋白-9的表达进行半定量分析。根据骨不连类型、患者年龄和骨不连时间对其进行统计学分析。结果:肥大性骨不连患者中转化生长因子β1和骨形态发生蛋白-9的吸光度值分别为0.3236±0.0390和0.1337±0.0400,萎缩性骨不连患者中转化生长因子β1和骨形态发生蛋白-9的吸光度值分别为0.3191±0.0369和0.1373±0.0423,两者标本中的吸光度值差异均无明显统计学意义(均P>0.05)。转化生长因子β1和骨形态发生蛋白-9在不同年龄、不同骨不连时间中的吸光度值差异也均无明显统计学意义(均P>0.05)。结论:肥大性骨不连组织和萎缩性骨不连组织在成骨潜能上无明显差异。

Objective： To examine the expression of transforming growth factor 1 （TGF-β1） and bone morphogenetic protein-9 （BMP-9） in human nonunion tissues, and to evaluate the clinical significance. Methods： The number of hypertrophic nonunion tissue samples and atrophic nonunion tissue samples were collected from Department of Orthopedics, the Second Xiangya Hospital of Central South University and Suzhou Kowloon Hospital Affiliated to School of Medicine of Shanghai Jiao Tong University between 2010 and 2014. Semi-quantification of SP immunohistochemical method and pathological image analysis software IPP6.0 were used to analyze the expression of TGF-β1 and BMP-9. Nonunion type, patients＇ age and nonunion time were statistical analyzed. Results： The absorbance values of TGF-β1 and BMP-9 in the hypertrophic nonunion tissues were 0.3236±.0390 and 0.1337±0.0400, respectively; while the absorbance values of TGF-β1 and BMP- 9 in the atrophic nonunion tissues were 0.3191±0.0369 and 0.1373±0.0423, respectively, with no significant difference between the two types of tissues （both P〉0.05）. There was also no significant difference in patients＇ age and bone nonunion time between them （all P〉0.05）. Conclusion： There is no significant difference in osteogenic potential between the hypertrophic nonunion tissues and the atrophic nonunion tissues.