钟花樱花粉离体萌发培养及低温保存技术研究

Pollen Germination in vitro Culture and Cryopreservation Technology of Cerasus campanulata

以钟花樱花粉为试材,采用离体萌发法检测花粉生活力,研究液体培养基中不同质量浓度蔗糖、硼酸、氯化钙对花粉萌发的影响,并在3种低温条件下进行花粉保存试验。结果表明:钟花樱花粉离体培养的最佳配方为蔗糖15%+硼酸50 mg/L+氯化钙100 mg/L。降低保存温度能延长钟花樱花粉保存时间,在4℃的环境条件下,保存30 d时检测花粉就完全失去活力;在-20℃的低温环境下,贮藏150 d时花粉萌发率为28.30%,270 d时降为0;在-80℃低温环境下,贮藏150 d时花粉萌发率为45.19%,270 d时降为0。

Abstract：The pollen of Cerasus campanulata were taken as material, aiming at studying the influence of pollen germination on different concentrations of sucrose, boric acid, calcium chloride by in vitro method. And the pollen preservation test was carried out under 3 kinds of low temperature conditions The result showed that the most suitable formula for pollen culture in vitro was 15% sucrose＋50mg/L boric acid＋100mg/L calcium chloride. Reducing the storage temperature can prolong the preservation of the pollen. Pollen almost dead after 30 days by stored in 4℃. The pollen germination rate was 2830% after 150 days by stored in -20℃, and dead after 270 days. The pollen germination rate was 4519% after 150 days by stored in -80℃, and dead after 270 days.