摘要
为寻找新的粘细菌次级代谢产物,分别采用滤纸片和大肠杆菌两种诱导法诱导粘细菌子实体的形成,挑取子实体多次转接以纯化菌株,从中筛选获得1株粘细菌CA3.通过菌体形态观察、生理生化试验和16S rDNA同源性分析的方法对其进行鉴定归类,确认其为叶柄粘球菌(Myxococcus stipitatus)新菌CA3.对粘球菌CA3发酵上清液进行细胞毒性试验,结果表明其对肿瘤细胞B16和SMMC-7721有很强的抑制效果,为后续的抗肿瘤活性组分结构解析和抗肿瘤机制的研究提供了重要基础.
In order to search for secondary metabolites of new myxobacteria, in this work, we induced the fruiting body by using filter paper induction and living Escherichia coli induction, and then harvested and inoculated into the culture medium several times to purify the myxobacteria. We finally obtained one new myxobacteria strain. By using the morphological observation, physiological and biochemical experiments as well as 16S rDNA homology analysis, we confirmed that it was Myxococcus stipitatus. The culture supernatant of this strain named CA3 had a highly effective toxicity against tumor cells B16 and SMMC-7721. This work provided preliminary basis for studying the antitumor compounds and antitumor mechanism of strain CA3. Our experimental results could provide important information for the preliminary development of new lead compounds as antitumor drugs.
出处
《湖南师范大学自然科学学报》
CAS
北大核心
2018年第1期24-29,共6页
Journal of Natural Science of Hunan Normal University
基金
2015湖南省大学生研究性学习和创新性实验计划资助项目(043-0040)
湖南省“生物发育工程及新产品研发协同创新中心”资助项目(20134486)