橄榄链霉菌CD5872产越霉素A的研究

Study on destomycin A produced by Streptomyces olivaceus CD5872

目的放线菌CD5872的分类鉴定及其活性次级代谢产物的研究。方法采用多相分类技术对菌株CD5872进行初步鉴定;菌株发酵液通过离子交换树脂和硅胶柱层析技术分离获得单体化合物CD-1。根据高分辨质谱、核磁共振谱图、天然产物数据库检索和高效液相分析确定活性化合物的结构。结果该菌株为橄榄网状链霉菌(Streptomyces olivoreticuli),化合物CD-1为越霉素A(destomycin A)。结论首次报道越霉素A的新产生菌,具有工业化开发价值。

Objective To isolate and identify the structures of active secondary metabolites from the culture broth of strain CD5872, and preliminarily identify its producing strain CD5872. Methods Strain CD5872 was identified by polyphasic taxonomy. Compound CD-1, identified as an active secondary metabolite, was purified by ion-exchange column chromatography and silica gel chromatography. The chemical structure of CD-1 was determined by HR-ESI-MS, NMR, HPLC analysis, and data comparisons with those reported in the related literatures. Results The strain CD5872 was affiliated with the genus Streptomyces olivoreticuli. The compound CD-1 was identified as destomycin A. Conclusion To our best knowledge, it is the first report which shows that destomycin A is produced by a Streptomyces olivoreticuli strain. Moreover, it is a safe and efficient insecticide and antibiotic, which has enormous economic values.