血脑屏障细胞体外培养模型研究进展

Research Progress in Cell-Culture Models of Blood-Brain Barrier

血脑屏障研究对神经系统疾病的药物研制有着重要的作用。细胞体外培养模型有助于研究脑微血管内皮细胞、星形胶质细胞、周细胞等血脑屏障内主要组成细胞的生理作用及生物特性,从而观测、分析给药过程中各部分的反应状况,优化给药过程,减弱毒性反应。根据血脑屏障细胞的特性,笔者介绍了现阶段较常用的单层模型、共培养模型和较为前沿的三维动力学模型等培养模型,并通过对各个模型优缺点的分析,提出了基于不同研究目的选择合适体外模型的建议。

Studies of blood-brain barrier （BBB） play an important role in the R＆D of new drugs for neurological disorders. Cellculture models of blood-brain barrier are helpful to study the functions and biological properties of the major BBB components, including brain microvascular endothelial cells, astrocytes, and pericytes, observe and analyze different components＇ reactions during the drug treatment, optimize the dosing progress, and reduce toxicity. According to the properties of individual BBB components, we sum- marized several widely used in vitro models of BBB, including monolayer models, co-culture models and the newly developed microflu- idic-based models. By comparing their advantages and disadvantages, we provided suggestions on the selection of BBB models accord- ing to different research purposes.