HPLC-ELSD同时测定知母药材中3种主要皂苷含量

Simultaneous determination of three major steroid glycosides in total saponins of Rhizoma Anemarrhenae by HPLC-ELSD

目的:首次建立同时测定知母药材中3种主要皂苷含量的HPLC-ELSD法。方法:采用Diamonsil C18(2)柱(250×4.6mm,5um),流动相为甲醇(A)-水(B),梯度洗脱;流速1.0m L·min^(-1);柱温30℃;蒸发光散射检测器雾化器流速2.0L·min^(-1);漂移管温度50℃;进样量10u L。结果:知母皂苷O、知母皂苷BII、知母皂苷BI浓度分别在64.13~1026μg·mL^(-1)(r=0.9989)、57.15~1028μg·mL^(-1)(r=0.9965)、67.32~1077μg·mL^(-1)(r=0.9998)的范围内呈良好的线性关系。加样回收率平均值为99.43%~102.8%,RSD为1.78%~2.58%。结论:该含量测定方法简便,准确,可靠,能同时测定知母皂苷O、知母皂苷BII、知母皂苷BI 3种有效成分含量。

Objective： To establish an HPLC-ELSD method for simuhaneous determination of three major steroid glycosides （Timosaponin O,Timosaponin BII and Timosaponin BI）.Method： The Diamonsil C18 （2）（ 250 mm×4.6 mm,5μm） column was used. The mobile phase consisted of methanol（A） and water（B） with gradient elu- tion at a flow rate of 1.0 mLomin-1.The column temperature was set at 30℃. The ELSD conditions were as follows： the nebulizing gas flow rate was 2.0 L·min-1, drift tube temperature was 50℃.The volume was 10μL.Result： The linearity was obtained over 64.13-1026 ug＇mL-1 （r=0.9989） for TimosaponinO,57.15-1028 ug＇mL-1（r=0.9965）for Timosaponin BII,67.32-1077ug·mL-1 （r=0.9998）for Timosaponin BI. The mean recoveries were between99.43%- 102.8%,RSD was 1.78%-2.58%.Conclusion：This method was quick and reliable which could simultaneously de- termine multiple steroid glycosides in total saponins of Rhizoma Anemarrhenae.