烟草内源提取物抗TMV活性筛选

Extracts from Different Tobacco Varieties with Inhibitory Activity against Tobacco Mosaic Virus ( TMV)

【目的】探讨3种烟草提取物的抗TMV活性,为开发烟草内源性抗烟草花叶病物质提供依据。【方法】利用半叶枯斑法对心叶烟、云烟203和K326三种烟草的根、茎、叶、杈甲醇提取物进行抗烟草普通花叶病毒活性筛选,并用叶盘法对部分筛选结果进行验证。【结果】3种烟草根和茎的提取物对TMV具有较明显的抑制活性,其中根对TMV体外钝化、初侵染和复制增殖作用的抑制率范围分别为65.00%~79.75%、64.0 8%~74.31%、26.9%~42.40%,活性最强;茎的活性次之,对TMV体外钝化、初侵染和复制增殖作用的抑制率范围分别为33.33%~44.25%、42.82%~66.06%、10.96%~47.76%。3种烟草叶和杈的提取物对TMV虽有一定的抑制活性,但较全株提取物对TMV体外钝化、初侵染和复制增殖作用的抑制活性均降低,抑制活性不明显。【结论】3种烟草的根部粗提物具有较好的抗TMV活性,值得进一步研究。

[ Purpose j The antiviral activities of tobacco extracts against tobacco mosaic virus （ TMV ） were studied to provide basis for exploitation of bio-pesticides from tobacco. [ Method] The inhibitory activity against tobacco mosaic virus （TMV） was testing respectively with extracts from roots, stems, leaves and branches of Nicotiana glutinosa, Yunyan 203 and K326 distilled by the half-leaf method, and leaf disk method were used to confirm the validity of the results. [ Result ] The results showed that the extracts fi＇om roots and stems of this three tobacco varieties showed a good inhibitory activity againstTMV. Especially, extracts from roots exhibited the best inhibitory activities and the ranges of inhibi- tion rates were 65.00% -79. 75%, 64. 08% -74.31% and 26. 9% -42.40% ; The inhibitory ac- tivities of the extracts from the stems were slightly weaker than the extracts from roots, but better than the extracts from leaves and branches, and the ranges of inhibition rates were 33.33% - 44.25%, 42. 82% -66.06% and 10. 96% -47.76%. The inhibition effect of the extracts from the leaves and branches of N. glutinosa, Yunyan 203 and K326 was not obvious. [ Conclusion ~ The extracts from roots of this three tobacco varieties show a good inhibitory activity against TMV, which is valuable to study more.