HPLC法测定滇芹中3种苯丙酸类成分的含量

Determination of Three Phenylalanine Components in Sinodielsia yunnanensis by HPLC

【目的】为比较滇芹两个主产地样品中的阿魏酸、咖啡酸及绿原酸的含量,建立HPLC法测定滇芹中3种苯丙酸类成分的HPLC分析方法。【方法】采用Waters Sun Fire C_(18)(150 mm×4.6 mm,3.5μm)色谱柱,以甲醇-0.1%甲酸水溶液为流动相,采用梯度洗脱,体积流量1 mL/min,柱温30℃,检测波长350 nm,进样量10μL。【结果】阿魏酸、咖啡酸、绿原酸分别在0.102~51、0.108~54、0.112~56μg/mL浓度范围内显示出良好的线性关系(r>0.999 0),平均回收率分别为98.15%、100.48%、101.25%,RSD为1.75%、1.69%、1.23%。【结论】应用该方法对西藏滇芹及云南滇芹进行了含量测定比较,西藏滇芹样品3种成分含量均低于云南滇芹。该方法简便、准确、可靠、重现性好,可用于滇芹药材中3种苯丙酸类成分的定量分析。

[ Purpose ] In order to compare the content of ferulic acid, caffeic acid and chlorogenic acid in Sinodielsia yunnanensis samples from two main origins, we established HPLC analysis method of three phen- ylalanine components. [ Method ] We used Waters SunFire C18 （ 150 mm × 4. 6 mm, 3. 5 μm） column with gradient elution consisting of methanol and 0. 1% formic acid in water at a flow rate of 1 mL/min. The column temperature was set at 30 ℃. The detection wavelength was 350 nm. The injection volume was 10 μL. [ Result] The standard curve of ferulic acid, caffeie acid, and chlorogenie acid respectively showed a good linearity （r 〉 0. 999 0） in the concentration range of 0. 102 -51, 0. 108 -54, 0. 112 -56 pg/mL, and the average recoveries were 98. 15%, 100. 48%, 101.25%, RSD were 1.75%, 1.69%, 1.23%. [ Conclusion] This method was used to comparing content detection of S. yunnanens/s of Tibet and S. yunnanens/s of Yunnan, the content of three components in Tibet celery were all lower than those in Yun- nan celery. The method is simple, accurate, reliable and reproducible, and can be used to do quantitative analysis of the three phenylalanine components in S. yunnanens/s herbs.