臭氧水浓度衰减及其杀菌作用

Attenuation rules and germicidal efficacy of ozoneted water

目的:检测在不同温度下产生的臭氧水的初始浓度以及室温(25℃)下臭氧水浓度的衰减规律,同时观察不同浓度臭氧水对各种常见病原微生物的杀灭效果。方法:采用HZ-2601B臭氧水疗仪制备不同温度的臭氧水,用在线检测法检测臭氧水浓度,平板培养法观察不同浓度臭氧水的杀菌效果。结果:水温为20,25,30,35及40℃时臭氧水疗仪产生的臭氧水的初始浓度分别为4.38,4.26,3.12,2.76及1.31 mg/L;0~10 min臭氧水浓度衰减速度较快;当臭氧水初始温度为25和30℃时,30 min后浓度分别为1.06和0.37 mg/L。平板试验结果显示浓度为1.0 mg/L臭氧水作用1 min对铜绿假单胞菌的杀灭率为99%,对其他常见菌的杀灭率为100%。浓度为0.3 mg/L臭氧水作用1 min对大肠杆菌、金黄色葡萄球菌、耐药金黄色葡萄球菌、铜绿假单胞菌及白色念珠菌的杀灭率分别为100%,100%,100%,95%和92%。结论:HZ-2601B型臭氧水疗仪所生成的臭氧水初始浓度随温度升高而降低;水温在20~30℃的臭氧水对常见病原菌具有较好的杀菌效果。

Objective： To determine initial concentrations of ozonated water under different temperatures, att enuation rules of ozonated water under the room temperature （25 ℃）, and to inspect the eff ects of ozonated water under diff erent concentrations on common microorganisms. Methods： Th e online test method and the plate cultivation method were employed to check the concentrations and killing rates on common microorganisms of ozonated water produced by HZ- 2601 B Ozone Water Generating Instrument. Results： The initial concentrations of ozonated water at 20, 25, 30, 35, and 40 ℃ were 4.38, 4.26, 3.12, 2.76, and 1.31 mg/L, respectively. The ozonated water was rapidly attenuated at first 10 min. The concentration of ozonated water still remained at 1.06 mg/L and 0.37 mg/L at 25 and 30 ℃ after 30 min. The average killing rates for Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, methicillin-resistant Staphylococcus aureus, and Candida albicans in 1.0 mg/L ozonated water for 1 min were 99%, 100%, 100%, 100%, and 100%, respectively. The average killing rates of Escherichia coli, Staphylococcus aureus, methicillin-resistant Staphylococcus aureus, Pseudomonas aeruginosa, and Candida albicans in 0.3 mg/L ozonated water for 1 min were 100%, 100%, 100%, 95%, and 92%, respectively. Conclusion： The initial concentrations of ozonated water produced by HZ-2601 B Ozone Water Generating Instrument decrease with the increase of temperature. Ozonated water under 20–30 ℃ has good sterilization effect on common microorganisms.