摘要
目的观察柚皮苷对心肌缺血/再灌注损伤(MI/R)大鼠血流动力学的影响,探讨其对MI/R大鼠的保护作用。方法采用结扎大鼠冠状动脉左前降支制备MI/R模型,同时给予不同剂量的柚皮苷(10、20、40mg/kg),7d后测定心电图,记录缺血前、缺血30min及再灌注不同时间点的左室舒张末期内压(LVEDP)、左室收缩内压(LVSP)、左室内压最大上升/下降速率(±dp/dtmax);化学比色法测定动脉血清肌酸激酶(CK)和乳酸脱氢酶(LDH)的活性。结果与MI/R组相比,柚皮苷20、40mg/kg可使LVEDP降低,±dp/dtmax和LVSP升高,血清中CK和LDH活性降低(P<0.05),呈现剂量依赖性。结论柚皮苷对大鼠MI/R具有保护作用,减轻心肌损伤程度。可能与改善血流动力学有关。
Objective To observe the effects of naringin （Nar） on hemodynamics in myocardial ischemia-reperfusion（MI/R） injury rats for investigating its protective role on MI/R rats. Methods The ischemia-reperfusion（I/R） model was prepared by liga2 ting the left anterior descending coronary artery,meanwhile different doses of Nat（10,20,40 mg/kg） were given. Then the electro- cardiogram （ECG） was recorded after 7 d. The left ventricular end diastolic pressure （LVEDP）.left ventricular systolic pressure （LVSP） and maximum ascending rate of left ventricular pressure/maximal descending rate of left ventricular pressure （± dp/dt-max） were measured. The activities of srum creatine kinase （CK） and lactate dehydrogenase （LDH） were measured by using the chemical colorimetry method. Results Compared with the MI/R group,Nar 20,40 mg/kg could decrease LVEDP,increased ±dp/ dtmax and LVSP,and decreased the activities of serum CK and LDH （P〈0.05）, showing a dose-dependent manner. Conclusion Nar has the protective effect against MI/R injury rat and alleviates the myocardial damage extent,which may be related with impro-ving the hemodynamics.
出处
《重庆医学》
CAS
2018年第6期733-735,739,共4页
Chongqing medicine
基金
重庆市教委科学技术研究项目(KJ1402501)
重庆市卫生和计划生育委员会中医药科技项目(ZY201703077)
重庆市万州区科学技术项目(201403051)
