处理高氨氮废水亚硝化细菌培养实验研究

Experimental Study on Nitrosobacteria Culture for High Ammonia-nitrogen Wastewater Treatment

为培养适应高氨氮废水短程硝化要求的亚硝化细菌,采用选择性传代培养及序批式定向培养对亚硝化细菌富集过程及影响因素进行研究,分析了水温、p H、溶解氧浓度等培养条件对亚硝化效果的影响。结果表明,富集的亚硝化细菌为短杆状亚硝化单胞菌,菌体大小为0.75μm×0.3μm,在水温T=(28±1)℃、p H=(7.6±1)、ρ(DO)=(1.2±0.2)mg/L的培养条件下,随着培养时间的延长和初始氨氮浓度的提高,亚硝化细菌逐渐适应了高氨氮水质环境,亚硝化细菌浓度达到2.4×10~9 CFU/m L,氨氧化速率达到21.8 mg/(L·h),亚硝酸氮累积率≥96.0%。

To culture nitrosobacteria for the shortcut nitrification of high ammonia-nitrogen wastewater, the enrichment process and influencing factors of nitrosobacteria were researched with selective subculture and sequencing directional culture. The influences of culturing conditions on nitrosation effects were analyzed, such as the water temperature, p H and dissolved oxygen concentration. The results showed that, the enriched nitrosobacteria belonged to nitrosomonas with the short-rod shape of 0.75 μm×0.3 μm. Under the conditions of water temperature, p H and ρ（DO） was（28±1）℃,（7.6±1） and（1.2±0.2）mg/L respectively, with the extension of culture time and the improvement of initial concentration of ammonia nitrogen, the nitrosobacteria gradually acclimatized to the wastewater with high ammonia nitrogen. The nitrosobacteria concentration reached 2.4 ×10^9 CFU/m L, the ammonia oxidation rate reached 21.8 mg/（L·h） and the Nitrite-N cumulative percentage was above 96.0%.