HCV基因型和rs11820062对丙型肝炎感染转归影响

Effects of HCV genotypes and V-Rel reticuloendotheliosis viral oncogene homolog A rs11820062 on outcome of HCV infected patients

目的探索丙型肝炎病毒(HCV)基因型和核转录因子-κB(NF-κB)家族V-Rel网状内皮增生病毒癌基因同源物A(RELA)基因上rs11820062位点对丙型肝炎患者感染转归影响。方法于2008年10月—2015年5月共收集788例丙肝感染者(其中有271例自限清除者和517例持续感染者),通过Taq Man-MGB探针法对788例丙肝患者的RELA基因上rs11820062位点基因分型,以Simmonds法对所有患者HCV基因型进行检测。用单因素和多因素logistic回归分析丙肝感染转归的高危因素。结果 271例HCV自限清除者中,HCV 1b型为146例(53.9%),非1b型为80例(29.5%),混合型为45例(16.6%);517例持续感染者中,HCV 1b型为234例(45.3%),非1b型为48例(9.3%),混合型为235例(45.4%)。单因素logistic回归分析结果显示,HCV基因型和HCV感染慢性化之间存在关联;多因素logistic回归分析结果显示,与HCV非1b基因型相比,1b型(OR=2.650,95%CI=1.631~4.307)和混合型(OR=3.159,95%CI=1.751~5.699)更易发展为慢性感染。在隐性模型中,携带有rs11820062 TT基因型(OR=1.689,95%CI=1.090~2.617)的患者存在慢性感染倾向。交互作用分析表明,HCV基因型和rs11820062之间对HCV感染慢性化的影响相互独立(P=0.280)。结论携带NF-κB家族rs11820062 TT基因型的丙肝感染者更易进展为慢性丙肝,且HCV基因型和rs11820062对HCV慢性化影响相互独立。

Objective To explore effects of genotype of hepatitis C virus （HCV） and rs11820062 of V-Rel reticu-loendotheliosis viral oncogene homolog A （RELA） of nuclear factor-kappa B （NF-κB） family genes on the outcome of HCV infection.Methods The HCV genotypes of 788 patients were genotyped with PCR products of 5＇ untranslated region （UTR） digested with restriction endonucleases. The rs11820062 was genotyped with TaqMan assay among the 788 HCV infected patients, including 271 spontaneous viral clearance subjects and 517 persistent HCV-infected subjects. Univariate and multivariate logistic regression analysis were used to analyze the association of relevant factors with HCV infection outcome.Results Of the 271 spontaneous viral clearance participants, 146 （53.9%）, 80 （29.5%）, and 45 （16.6%） were identified with the HCV genotype of 1b, non-1b, and the mixed; of the 517 persistent HCV-infected participants, 234 （45.3%）, 48 （9.3%）, and 235 （45.4%） were identified with the HCV genotype of 1b, non-1b, and the mixed. Univariate logistic regression analysis showed that HCV genotype was correlated with the chronicity of HCV infection. The results of multivariage logistic regression analysis showed that the participants with the HCV infection of 1b （odds ratio [OR] = 2.650, 95% confidence interval [95% CI]： 1.631-4.307） or the mixed genotype （OR = 3.159, 95% CI： 1.751-5.699） were more likely to have chronic HCV infection compared to those with the HCV infection of non-1b genotype. Based on a recessive model, the participants carrying TT genotype of rs11820062 were prone to develop chronic HCV infection. Interactive analysisshowed that the effects of HCV genotype and rs11820082 on the outcome of HCV infection are independent （P = 0.280）.Conclusion The HCV infected individuals with TT genotype of rs11820082 of NF-κB family genes are prone to develop chronic HCV infection and the effects of HCV genotype and genotype of rs11820082 on the chronicity of HCV infection are self-independent.