二仙汤抗骨质疏松有效组分对维甲酸致骨丢失大鼠的影响

Effect of antiosteoporotic active fraction from Er-Xian Decoction on retinoic acid-induced bone loss in rats

目的探讨二仙汤抗骨质疏松有效组分对维甲酸致骨质疏松大鼠的影响。方法将60只雌性SD大鼠随机分为空白对照组、模型组、戊酸雌二醇(0.2 mg/kg,阳性对照)组和二仙汤抗骨质疏松有效组分低、中、高剂量(50、100、200 mg/kg)组。除空白对照组外,各组大鼠连续14 d灌胃给予维甲酸[70 mg/(kg·d)]诱导骨质疏松症。造模结束后,按分组设计方案对各组大鼠每天灌胃给药(10 m L/kg),连续28 d。用ELISA试剂盒检测大鼠血清和尿液的生物化学指标,显微CT分析股骨远端骨密度和骨小梁微结构的变化。结果二仙汤抗骨质疏松有效组分可降低维甲酸致骨质疏松大鼠尿液钙的含量,以及血清碱性磷酸酶和抗酒石酸酸性磷酸酶的活性(P<0.05或P<0.01),增加骨密度(高剂量组P<0.05),改善骨小梁微结构的退化。结论二仙汤抗骨质疏松有效组分可减少维甲酸致骨质疏松大鼠的骨丢失和骨组织微结构的退化。

Objective To explore the effect of antiosteoporotic active fraction from Er-Xian Decoction（EXD） on retinoic acid-induced osteoporosis in rats. Methods Sixty female SD rats were randomly divided into normal control group, model group, estradiol valerate（0.2 mg/kg） group and antiosteoporotic active fraction from EXD low-, middle-and highdose groups（50, 100, 200 mg/kg）. Except for the rats of normal group, the others were intragastrically treated with 70 mg/（kg · d）of retinoic acid for 14 days to induce osteoporosis. Then the rats were treated with estradiol valeratse or antiosteoporotic active fraction from EXD（10 m L/kg） for 28 days. The biochemical parameters in serum and urine of rats were detected by ELISA. The bone mineral density and trabecular bone microstructure of distal femur were analyzed with micro-CT. Results The antiosteoporotic active fraction from EXD significantly decreased calcium content in urine and activities of alkaline phosphatase and tartratse-resistant acid phosphatase in the sera of retinoic acid-induced osteoporotic rats（P0.05, P0.01）. High-dose of antiosteoporotic active fraction from EXD significantly increased bone mineral density of distal femur（P0.05）. And the antiosteoporotic active fraction from EXD improved the trabecular bone microstructure of distal femur in retinoic acid-induced osteoporotic rats. Conclusion Antiosteoporotic active fraction from EXD can reduce bone loss and deterioration of bone microstructure in retinoic acid-induced osteoporotic rats.