特应性皮炎患者嗜酸性粒细胞富集群中P物质及其受体NK1R的表达变化

Expressions of SP and NK1R in eosinophil-enriched blood cells from patients with atopic dermatitis

目的检测特应性皮炎(AD)患者外周血嗜酸性粒细胞富集群中P物质(SP)和NK1受体(NK1R)的表达,并分析其在AD中的可能作用。方法采集健康对照组(HC)及AD患者的外周静脉血,用尘螨、蒿草花粉和梧桐花粉过敏原粗提液刺激1h后,流式细胞术检测嗜酸性粒细胞富集群中SP和NK1R的表达。结果与HC组比较,静息状态下AD患者嗜酸性粒细胞富集群中NK1R^+细胞的比例升高了41%(P=0.001),SP^+细胞的比例减少了1.17倍(P<0.001),SP^+细胞的MFI降低了55%(P<0.001)。过敏原对HC和AD患者嗜酸性粒细胞富集群中SP及NK1R表达的影响无统计学差异。结论 AD患者嗜酸性粒细胞富集群中NK1R表达上调,提示嗜酸性粒细胞表面的NK1R可能在特应性皮炎中起重要作用;NK1R拮抗剂或阻断剂可能是治疗AD的有效制剂。

Objective To investigate the expressions of substance P(SP)and neurokinin receptor(NK1 R)in eosinophil-enriched cells from patients with atopic dermatitis(AD)so as to elucidate their possible roles in AD.Methods Blood samples were collected from healthy controls(HCs)and AD patients,and incubated with the extracts of Artemisia pollen,dust mite,and Platanus pollen for 1 h.The expressions of SP and NK1 R in eosinophilenriched cells were detected by flow cytometry.Results Level of NK1 R in eosinophil-enriched cells from AD patients increased by 41% compared with that of HCs when cultured with the medium only(P =0.001).In addition,the expression of SP in AD patients decreased by 1.17 folds(P<0.001),and mean fluorescence intensity(MFI)of SP^+eosinophils decreased by 55%(P<0.001).However,allergens had little effect on the expressions of SP and NK1 R in eosinophil-enriched cells from AD patients and HCs.Conclusion Upregulated expression of NK1 R in AD indicates that eosinophil-derived NK1 R may play an important role in AD.Antagonists or blockers of NK1 R might be effective preparations for AD treatment.