基于基因组学探讨糖尿病肾病的发病原因及其与心血管疾病的关系

Mechanism of diabetic kidney disease and its relationship with cardiovascular disease based on genomics

目的分析糖尿病肾病的发病原因及其与心血管疾病发生的关系,为二者的早期诊断和临床治疗提供依据。方法从Pubmed基因芯片公共数据库(GEO Database)中下载糖尿病肾病基因芯片数据,将数据导入R语言、QOE、STRING、GCBI及GenClip等分析软件或数据库,分析健康对照组与糖尿病肾病组的基因表达谱、基因功能、蛋白质相互作用网络、基因及信号共表达网络等,筛选两组之间的关键节点基因。结果糖尿病肾病患者肾小球组织的基因表达谱有明显改变;与健康人群相比,糖尿病肾病患者肾小球组织有844个差异表达基因;前20个差异表达基因蛋白-蛋白相互作用分析发现1个以TNNT2为核心的明显的蛋白相互作用网络;差异表达基因主要与炎症反应、应激反应等有关,且与MAPK信号通路关系密切。结论糖尿病肾病的发病原因主要与炎症反应、应激反应相关,且与心血管疾病的发生有一定的关系。

Objective To provide the basis for early diagnosis and clinical treatment for diabetic kidney disease(DKD)and cardiovascular disease by analyzing the causes of DKD and the relationship between them.Methods Microarray gene chip data from the glomerulus in DKD were downloaded from GEO Database.The gene expression profile,gene function,protein-protein interaction network,gene and signal pathway co-expression network of control and DKD groups were analyzed and the key node genes between the two groups were selected by R,QOE,GCBI,STRING and Genclip.Results The gene expression profile of glomerular tissue in DKD patients was significantly altered.Compared with control group,there were 844 differentially expressed genes in DKD patients.One protein-protein interaction network with the core of TNNT2 occurred among top 20 differentially expressed genes.Differentially expressed genes were mainly related to inflammatory and stress responses,as well as to MAPK signal pathway.Conclusion The cause of DKD is mainly related to inflammatory and stress responses,which are also related to cardiovascular disease.