非梗阻性无精子症患者姐妹染色单体互换频率的观察

Observation on the frequency of sister chromatid exchange in patients with non-obstructive azoospermia

目的探讨外周血淋巴细胞姐妹染色单体互换(SCE)频率与非梗阻性无精子症(NOA)形成的相关性,为进行病因学研究和采取干预措施打下基础。方法对30例NOA患者及20例已经生育的男性,按照外周血淋巴细胞SCE检测方法进行培养检测,分别计数SCE频率。结果实验组SCE频率为8.39±1.58%;对照组为5.17±0.53%,两组对比差异非常显著(P<0.01)。结论 NOA患者的外周血淋巴细胞SCE频率明显增高,表明其染色体具有不稳定性。

Objective：To investigate the correlation between the frequency of sister chromatid exchange（SCE）and the formation of non-obstructive azoospermia（NOA）in peripheral blood lymphocytes,and to lay the foundation for the study of etiology and intervention.Methods：Thirty patients with NOA and 20 males who had been born were examined by SCE detection method.The frequency of SCE was counted respectively.Results：The frequency of SCE was 8.39±1.58% in the experimental group and 5.17±0.53% in the control group.The difference between the two groups was significant（P〈0.01）.Conclusion：The frequency of SCE in peripheral blood lymphocytes of NOA patients is significantly higher,indicating that the chromosomes are unstable.