不同Gn剂量对IVF-ET胚胎结果影响的研究

Effects of different doses of Gn on IVF-ET embryos

目的采取回顾性病例对照的研究方法,观察不同剂量Gn对体外受精-胚胎移植(IVF-ET)患者胚胎结果的影响。方法对243名因单纯输卵管因素行IVF长方案助孕患者新鲜周期胚胎结果进行回顾性分析,其中单纯用HMG进行COH32例(A组),其中Gn<2000IU(A1组)1例,2000<Gn≤3000(A2组)6例,3000<Gn≤4000(A3组)14例,4000<Gn≤5000(A4组)8例,GN≥5000(A5组)3例:使用HMG+普利康进行COH77例(B组),Gn<2000IU(B1组)6例,其中2000<Gn≤3000(B2组)23例,3000<Gn≤4000(B3组)31例,4000<Gn≤5000(B4组)10例,GN≥5000(B5组)7例:使用HMG+果纳芬进行COH133例(C组),其中Gn<2000IU(C1组)13例,2000<Gn≤3000(C2组)79例,3000<Gn≤4000(C3组)31例,4000<Gn≤5000(C4组)8例,GN≥5000(C5组)2例,对比A、B、C组内不同Gn剂量对胚胎结果情况。结果在A组内,A5组2PN卵裂率高于其他组,差异有统计学意义(P<0.05),A5组受精率高于可移植胚率高于其他组,A2组可移植胚率高于其他组、A2组优胚率高于其他组、但差异均无统计学意义(P>0.05);在B组内,B5组受精率高于A1,A2,A3,A4组,差异有统计学意义(P<0.05)、B4组优质胚胎率高于A1,A2,A3,A5组,差异有统计学意义(P<0.05),B4组2PN卵裂率高于其他组、B5组可移植胚率高于其他组,但差异均无统计学意义(P>0.05);在C组内,C5组受精率高于其他组,差异有统计学意义(P<0.05),C5组2PN卵裂但率高于其他组、C5组可移植胚率高于其他组、C1组优质胚胎率高于其他组,但差异均无统计学意义(P<0.05)。结论对于IVF患者,使用不同Gn剂量可对胚胎结局产生不同影响,但Gn剂量与局并非呈正相关。

Objective：To observe the effect of different doses of Gn on the embryonic outcome of IVF-ET patients in retrospective follow-up study.Methods：A retrospective analysis of 243 patients with IVF long regimen was studied retrospectively.The results showed that there were 1 case of Gn2000 IU（A1 group）,2000Gn≤ 3000）,8 cases of 4000Gn≤4000（A3 group）,8 cases of 4000Gn≤5000（A4 group）,and 3 cases of GN≥5000（A5 group）（Group B）,Gn2000 IU（group B1）6 cases,of which39 cases of29 2000Gn ≤ 3000（B2 group）23 cases,3000Gn ≤ 4000（B3 group）31 cases,4000Gn ≤ 5000（B4 group）10（Group C）,including Gn2000 IU（C1 group）in 13 cases,2000Gn ≤ 3000（C2 group）79 cases,39 cases of 3000Gn≤4000（C3 group）,8 cases of 4000Gn≤5000（C4 group）,2 cases of GN≥5000（C5 group）,and the results of different Gn dose in the A,B and C groups were compared.Results：In group A,the cleavage rate of 2 PN in A5 group was higher than that in other groups（P〈0.05）.The fertilization rate of A5 group was higher than that of other group,（P〈0.05）.In group B,the fertilization rate in group B was higher than that in group A1,A2,A3 and A4,and the difference was significant（P〈0.05）（P〈0.05）.The rate of high-quality embryos in B4 group was higher than that in A1,A2,A3 and A5 patients（P〈0.05）.The cleavage rate of BP group was higher than that of other group（P〈0.05）.In the C group,the fertilization rate of C5 group was higher than that of other groups,the difference was statistically significant（P〈0.05）,C5 group 2 PN egg was not statistically significant（P〈0.05）（P〈0.05）,but the difference was not statistically significant（P〈0.05）,but the difference was not significant（P〈0.05）.Conclusions：For patients with IVF,the use of different Gn doses may have different effects on the outcome of the embryo,but the Gn dose is not positively related to the outcome.