犊牛腹泻大肠埃希菌O-抗原血清型鉴定

Identification of serogroup of E. coli O-antigen from calves with diarrhea

目的对犊牛腹泻大肠埃希菌O-抗原血清型进行鉴定。方法收集内蒙古部分地区牛场患犊牛腹泻病的犊牛直肠内容物或粪便共165份,经细菌分离培养、动物致病性试验及16s RNA PCR检测,筛选出致病性大肠埃希菌,并进行O-抗原血清型鉴定。结果分离的大肠埃希菌共165株,115株有致病性,除7株未能确定分型及3株自凝集外,其余105株鉴定为O-抗原血清型。结论大肠埃希菌O-抗原血清型鉴定对流行病学调查及疾病预防均有重要意义。

Objective To identify the serogroup of E. coli O-antigen from calves with diarrhea. Methods A total of 165 samples of rectal contents or feces were collected from calves with diarrhea in cattle farms of partial regions in Inner Mongolia Autonomous Region, China, from which the pathogenic E. coli were screened by bacterial isolation and culture,pathogenicity test in animals and 16 s RNA PCR, and subjected to serogrouping of O-antigen. Results Of the 165 E. coli isolates, 115 were pathogenic. Except 7 isolates with undefined serogroups and 3 with auto-aggregation, the remained 105 isolates were identified as the serogroups of O-antigen. Conclusion The identification of serogroup of E. coli O-antigen was important to epidemiological investigation and prevention of diseases.