金魁猕猴桃RT-qPCR内参基因的筛选

Screening of reference genes for reverse transcription quantitative real-time PCR in Actinidia deliciosa

以'金魁'猕猴桃(Actinidia deliciosa)根、茎、叶、花瓣、花萼、雌蕊、子房、幼果为材料,应用实时荧光定量PCR(RT-qPCR)技术分析了ACT、CYP2、RP2、GAPDH、TUB和TUA 6个常用内参基因在猕猴桃不同器官组织中的表达情况,并利用GeNorm、NormFinder和BestKeeper软件对候选内参基因的稳定性进行了评价。结果表明:ACT和TUA基因在各组织中的表达量差异较小,表达相对稳定;在利用RT-qPCR分析比较猕猴桃不同器官组织中的基因表达差异时,可选择ACT作为内参基因。

Taking the root,stem,leaf,calyx,petal,pistil,ovary and fruitlet of Actinidia delicious＇ Jinkui＇ as materials,we analyzed the expression of 6 commonly used reference genes（ACT,CYP2,RP2,GAPDH,TUB and TUA）in different organs and tissues of Actinidia by RT-qPCR technology,and evaluated the stability of candidate reference genes by GeNorm, NormFinder and BestKeeper software. The results showed that the difference in expression of ACT and TUA genes in each tissue were relatively small,and the expression were relatively stable.In the analysis and comparison of gene expression in different kiwifruit organs and tissues by RT-qPCR,ACT could be chosen as the reference gene.