GRIN1、GRIN2B基因多态性与氯胺酮依赖的关联研究

Correlation between GRIN1,GRIN2B Gene Polymorphism and ketamine dependence

目的:探讨中国汉族人群N-甲基-D-天门冬氨酸(NMDA)受体亚基NMDA1基因(GRIN1)和NMDA2B基因(GRIN2B)多态性是否与氯胺酮成瘾存在关联。方法:采用聚合酶链式反应(PCR)及限制性片段长度多态性(RFLP)技术检测113例氯胺酮依赖者和136例正常对照的GRIN1、GRIN2B基因多态性,采用病例—对照的关联分析方法对基因型和等位基因频率进行分析。结果:患者组和对照组的GRIN1、GRIN2B基因型符合Hardy—Weinberg平衡法则;两组间GRIN1、GRIN2B基因型和等位基因频率分布均无显著性差异(p≥0.05)。结论:GRIN1、GRIN2B基因型和等位基因频率分布与氯胺酮依赖可能无关联性。

Objective：To investigate the correlation between N methyl D aspartate（NMDA） receptor subunit,NMDA1 gene（GRIN1）,NMDA2 B gene（GRIN2 B） gene polymorphism and ketamine dependence in Chinese Han population. Methods：The polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP） technique was used to detected gene polymorphism of GRIN1、GRIN2 B in 113 ketamine dependent subjects and 136 health controls. Genotype and allele frequencies were analyzed by case-control analysis. Results：The GRIN1,GRIN2 B genotype of patients and controls was in accordance with the Hardy—Weinberg balance rule; There was no significant difference in the GRIN1,GRIN2 B genotype and allele frequency distribution between the two groups（ p≥0.05）. Conclusion：The GRIN1,GRIN2 B genotype and allele frequency distribution maybe were not associated with ketamine dependence.