益气健脾通便方对慢传输型便秘大鼠Cajal间质细胞及一氧化氮、一氧化氮合酶的影响

Effect of qi-tonifying,spleen-strengthening,and bowel-relaxing prescription on Cajal interstitial cells,nitric oxide,and nitric oxide synthase in rats with slow transit constipation

目的通过观察益气健脾通便方对慢传输型便秘(slow transit constipation,STC)大鼠Cajal间质细胞(Cajal interstitial cells,ICC)和一氧化氮(nitric oxide,NO)、一氧化氮合酶(nitric oxide synthase,NOS)的影响,探讨其作用机制。方法将60只SPF级雄性8周龄Wistar大鼠随机分为正常组,模型组,益气健脾通便方低、中、高剂量组和莫沙必利组,每组10只。采用"复方地芬诺酯灌胃法"复制STC大鼠模型。模型复制后,各组大鼠连续给药4周,观察每组大鼠的一般情况,测定粪便粒数、粪便含水率、肠道推进率和结肠组织ICC表达量及NO、NOS含量。结果益气健脾通便方能改善大鼠的一般情况,提高大鼠的粪便粒数、粪便含水率和肠道推进率(P<0.05),提高结肠组织ICC表达量(P<0.05),减少神经递质NO、NOS的含量(P<0.05)。结论 STC的发病机制可能与ICC表达量降低及NO、NOS水平升高有关。

Objective To investigate the effect of qi-tonifying,spleen-strengthening,and bowel-relaxing prescription on Cajal interstitial cells(ICC),nitric oxide(NO),and nitric oxide synthase(NOS)in rats with slow transit constipation(STC)and possible mechanism of action.Methods A total of 60 specific pathogen-free male Wistar rats aged 8 weeks were randomly divided into normal group,model group,low-,medium-,and high-dose qi-tonifying,spleen-strengthening,and bowel-relaxing prescription groups,and mosapride group,with 10 rats in each group.Compound diphenoxylate was given by gavage to establish a rat model of STC.After the model was established,the drugs were given for 4 consecutive weeks.The general status of rats was observed,and the number of fecal pellets,fecal water content,intestinal propulsion rate,expression of ICC,and content of NO and NOS were measured.Results The qi-tonifying,spleen-strengthening,and bowel-relaxing prescription improved the general status of rats,increased the number of fecal pellets,fecal water content,intestinal propulsion rate,and the expression of ICC in the colon tissue,and reduced the content of neurotransmitters NO and NOS(all P<0.05).Conclusion The pathogenesis of STC may be associated with the reduction in the expression of ICC and the increase in the levels of NO and NOS.