卵巢癌组织中LSD1、NDRG1基因表达量与癌细胞迁移、侵袭的相关性研究

The correlation of LSD1 and NDRG1 gene expression in ovarian cancer tissue with cancer cell migration and invasion

目的:研究卵巢癌组织中赖氨酸特异性组蛋白去甲基化酶1(lysine-specific demethylase 1,LSD1)、N-myc下游调节基因1(N-myc downstream regulated 1,NDRG1)基因表达量与癌细胞迁移、侵袭的相关性。方法:选择2014年3月~2017年7月扶风县人民医院接受手术切除的卵巢癌患者作为研究对象,手术切除后取卵巢癌组织和癌旁组织,测定LSD1、NDRG1基因及迁移基因、侵袭基因的表达量。结果:卵巢癌组织中LSD1、YKL40、COX2、Twist、IFITM1、CatL、CTHRC1、MMP2、FUNDC1基因的mRNA表达量显著高于癌旁组织,NDRG1、E-cadherin、Wnt5a基因的mRNA表达量显著低于癌旁组织;LSD1高表达的卵巢癌组织中YKL40、COX2、Twist、IFITM1、CatL、CTHRC1、MMP2、FUNDC1的mRNA表达量显著高于LSD1低表达的卵巢癌组织,E-cadherin、Wnt5a基因的mRNA表达量显著低于LSD1低表达的卵巢癌组织。结论:卵巢癌组织中LSD1高表达、NDRG1低表达能够促进癌细胞的迁移、侵袭。

Objective:To study the correlation of LSD1 and NDRG1 gene expression in ovarian cancer tissue with cancer cell migration and invasion.Methods:Patients with ovarian cancer who underwent surgical resection in Fufeng County People's Hospital between March 2014 and July 2017 were selected as the research subjects,and the ovarian cancer tissue and adjacent tissue were collected after surgical resection to determine the expression of LSD1,NDRG1,migration genes and invasion genes.Results:LSD1,YKL40,COX2,Twist,IFITM1,CatL,CTHRC1,MMP2 and FUNDC1 mRNA expression in ovarian cancer tissue were significantly higher than those in adjacent tissue whereas NDRG1,E-cadherin and Wnt5 amRNA expression were significantly lower than those in adjacent tissue;YKL40,COX2,Twist,IFITM1,CatL,CTHRC1,MMP2 and FUNDC1 mRNA expression in ovarian cancer tissue with high LSD1 expression were significantly higher than those in ovarian tissue with low LSD1 expression whereas E-cadherin and Wnt5 agene mRNA expression were significantly lower than those in ovarian tissue with low LSD1 expression.Conclusion:The high LSD1 expression and low NDRG1 expression in ovarian cancer tissue can promote the migration and invasion of cancer cells.