食管癌术前VEP新辅助化疗对肿瘤细胞恶性生物学行为的影响

Effects of VEP neoadjuvant chemotherapy before esophageal cancer surgery on the malignant biological behaviors of tumor cells

目的:研究食管癌术前VEP新辅助化疗对肿瘤细胞恶性生物学行为的影响。方法:选择2015年3月~2017年3月当阳市人民医院诊断为局部晚期食管癌的患者作为研究对象,随机分为接受术前VEP方案(依托泊苷+5-氟尿嘧啶+顺铂)化疗的VEP组、接受常规术前准备的对照组。确诊时及手术前1d,测定血清中肿瘤标志物的含量;手术切除后,测定肿瘤组织中增殖基因、侵袭基因的表达量。结果:手术前1d,VEP组患者血清中CEA、CA125、CYFRA21-1、SCC-Ag的含量均显著低于确诊时(P<0.05),对照组患者血清中CEA、CA125、CYFRA21-1、SCC-Ag的含量与确诊时比较无显著性差异(P>0.05);手术切除后,VEP组肿瘤组织中PTEN、Smac、PTPN14的mRNA表达量显著高于对照组(P<0.05),CyclinB1、CDK1、Grp94、MMP2、β-catenin、Slug、Vimentin、N-cadherin的mRNA表达量显著低于对照组(P<0.05)。结论:食管癌术前VEP新辅助化疗能够降低肿瘤细胞的生长活力并抑制病灶内肿瘤细胞的增殖、侵袭。

Objective:To study the effects of VEP neoadjuvant chemotherapy before esophageal cancer surgery on the malignant biological behaviors of tumor cells.Methods:Patients who were diagnosed with locally advanced esophageal cancer in Dangyang People's Hospital between March 2015 and March 2017 were selected as the research subjects and randomly divided into the VEP group who received preoperative VEP(etoposide + 5-fluorouracil+ cisplatin)chemotherapy and the control group who accepted routine preoperative preparation.The serum contents of tumor markers were determined at diagnosis and 1 day before surgery;the expression of proliferation genes and invasion genes in tumor tissue were determined after surgical resection.Results:1 day before surgery,serum CEA,CA125,CYFRA21-1 and SCC-Ag levels of VEP group were significantly lower than those at diagnosis,and serum CEA,CA125,CYFRA21-1 and SCC-Ag levels of control group were not significantly different from those at diagnosis;after surgical resection,PTEN,Smac and PTPN14 mRNA expression in the tumor tissue of VEP group were significantly higher than those of control group whereas CyclinB1,CDK1,Grp94,MMP2,β-catenin,Slug,Vimentin and N-cadherin mRNA expression were significantly lower than those of control group.Conclusion:VEP neoadjuvant chemotherapy before esophageal cancer surgery can reduce the growth of tumor cells and inhibit the proliferation and invasion of tumor cells in the lesion.