ⅠB～ ⅡB期宫颈癌患者术前新辅助化疗对病灶内恶性生物学特征的影响

Effects of preoperative neoadjuvant chemotherapy on malignant biological characteristics in the lesions of patients withⅠB-ⅡB cervical cancer

目的:研究ⅠB~ⅡB期宫颈癌患者术前新辅助化疗对病灶内恶性生物学特征的影响。方法:选择2015年2月~2017年5月海军总医院诊断为ⅠB~ⅡB期宫颈癌并接受手术治疗的患者作为研究对象,随机分为术前接受新辅助化疗的辅助化疗组和进行常规术前准备的对照组。手术切除后,通过荧光定量PCR测定宫颈癌组织中抑癌基因、增殖基因、迁移和侵袭基因的mRNA表达量。结果:手术切除后,辅助化疗组宫颈癌组织中抑癌基因THBS2、PTEN、LAST1、eIF4E3的mRNA表达量显著高于常规手术组,增殖基因RUNX2、CyclinD1、ALEX1、ALDH1、ABCG2及迁移和侵袭基因CXCL12、CXCR4、MMP9、S100A6、N-cadherin的mRNA表达量显著低于常规手术组。差异均有统计学意义(P<0.05)。结论:ⅠB~ⅡB期宫颈癌患者术前新辅助化疗能够抑制病灶内癌细胞的增殖、迁移和侵袭。

Objective:To study the effects of preoperative neoadjuvant chemotherapy on malignant biological characteristics in the lesions of patients withⅠB-ⅡB cervical cancer.Methods:Patients who were diagnosed withⅠB-ⅡB cervical cancer and underwent surgical treatment in Navy General Hospital of PLA between February 2015 and May 2017 were selected as the research subjects and randomly divided into the neoadjuvant chemotherapy group who underwent preoperative neoadjuvant chemotherapy and the control group who underwent routine preoperative preparation.After surgical resection,the mRNA expression of tumor suppressor genes,proliferation genes as well as migration and invasion genes in cervical cancer tissue were determined by fluorescence quantitative PCR.Results:After surgical resection,tumor suppressor genes THBS2,PTEN,LAST1 and eIF4 E3 mRNA expression in cervical cancer tissue of adjuvant chemotherapy group were significantly higher than those of control group whereas proliferation genes RUNX2,CyclinD1,ALEX1,ALDH1 and ABCG2 as well as migration and invasion genes CXCL12,CXCR4,MMP9,S100 A6 and N-cadherin mRNA expression were significantly lower than those of control group.Conclusion:Preoperative neoadjuvant chemotherapy can inhibit the proliferation,migration and invasion of cancer cells within the lesions of patients with ⅠB-ⅡB cervical cancer.