诺丽果粉水溶液对MCF-7人乳腺癌细胞及其内源性VEGF影响

The Effects of Noni's Freeze-Dried Powder on Breast Cancer MCf-7 Cell Line and Endogenous VEGF by Tumor Cells

用诺丽冻干粉加入至不完全培养基中,培养人乳腺癌Mcf-7细胞,通过Cck-8、流式细胞术、TUNELDAPI染色等方法体外实验,比较不同处理方式下细胞的多方面生物学特性.Cck-8检测法的结果显示,经诺丽果粉水溶液处理细胞的Cck-8吸光值低于用不完全培养基培养细胞的吸光值,并且与作用时间呈负相关性.TUNEL-DAPI双染结果显示,经诺丽果粉水溶液处理的细胞凋亡率高于正常培养的细胞,并且凋亡率与浓度呈正相关性.流式细胞术检测结果显示,经过诺丽果粉水溶液处理的细胞在1 mg·m L^(-1)浓度下,24 h即会发生早期凋亡.酶联免疫吸附(Elisa法)检测内源性VEGF含量结果显示,经诺丽果粉水溶液处理后的细胞上清液中VEGF的含量:20,10 mg·m L^(-1)浓度下VEGF含量降低,5,2,1 mg·m L^(-1)浓度下VEGF含量升高.诺丽果粉水溶液对Mcf-7人乳腺癌细胞系有生长抑制作用,作用方式可能为阻碍VEGF与细胞结合.

Adding nonius freeze-dry powder into Incomplete medium to culture Mcf-7 cell. This paper will compare cellular biological characteristics,including Cck-8,flow cytometry, TUNEL-DAPI staining and so on. Cck-8 assay showed that the Cck-8 absorption value of the cells treated with aqueous solution of Noni fruit powder is lower than those with incomplete cultured cell light absorption value,and it has negative correlation with time. TUNEL-DAPI double staining showed that the apoptosis rate of the normal cell apoptosis is higher than the one with Noni powder aqueous solution treatment, and the apoptosis rate was positively correlated with the concentration. Flow cytometry results showed that the cells treated with Noni powder aqueous solution in the concentration of 1 mg ？ mL ~1, would occurs early apoptosis within 24 h. Enzyme linked immunosorbent assay （Elisa method） to detect endogenous VEGF content showed that the content of VEGF reduced after treated in aqueous solution with Noni powder of 20 mg·mL^-1 and 10 mg·mL^-1,but VEGF content increased after treated in aqueous solution with Noni powder of 5 mg·mL^-1,2 mg·mL^-1 and 1 mg·mL^-1 concentration. Noni powder solution inhibited the growth of Mcf-7 human breast cancer cell,and the mechanism might be by combining suppressor cell with VEGF.