摘要
CRISPR(Clustered regularly interspaced short palindromic repeats,规律成簇的间隔短回文重复)平台是出色的基因组编辑工具,其中新型核酸酶Cas13a(CRISPR-associated protein 13a)既可编辑DNA,也可编辑RNA,这一新平台可实现DNA或RNA的摩尔灵敏度以及单碱基错配的特异性检测,同时拓展用于病毒与细菌的精细检测(甚至是寨卡与登革热的近亲病毒株)、癌症早期监测和遗传性疾病等治疗;其需要设计少,技术操作简单,具有广泛的应用潜力,是基因组编辑的又一项卓越成果。综述了Cas13a系统的最新进展。
The CRISPR（clustered regularly interspaced short palindromic repeats） platform is an excellent genome editing platform, in which, the novel nuclease Cas13 a（CRISPR-associated protein 13 a） can edit DNA and RNA. This new platform could fulfill DNA or RNA detection with molar sensitivity and single-base mismatch specificity, and meanwhile be extended to fine detect of viruses（even Close relatives of Zika and Dengue strains） and bacteria, monitor early cancer, with the simple technical manipulation and a wide range of application potentials, which could be another excellent tool of genomic editing.Here the current progress about Cas13 a system was overviewed.
出处
《湖北农业科学》
2018年第2期5-8,共4页
Hubei Agricultural Sciences
基金
国家自然科学基金(31640077)
湖北省自然科学基金重点项目(2016CFA072)
湖北省农业科技创新中心创新团队项目(2016-620-000-001-022)
